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Lab Exercise 17:

Lab Exercise 17:. Biochemical Differentiation of some Medically Important Gram-negative Bacilli. MacConkey Agar. Left: Shigella - result Right: Escherichia + result. Carbohydrate Fermentation Tests. Glucose w/ Durham Fermentation Tube. Carbohydrate Fermentation Tests.

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Lab Exercise 17:

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  1. Lab Exercise 17: Biochemical Differentiation of some Medically Important Gram-negative Bacilli

  2. MacConkey Agar Left: Shigella - result Right: Escherichia + result

  3. Carbohydrate Fermentation Tests Glucose w/ Durham Fermentation Tube

  4. Carbohydrate Fermentation Tests Sucrose Fermentation

  5. -/+/+ Triple Sugar Iron

  6. broth with tryptophan IMViC Series Indole Test

  7. IMViC Series Methyl Red Test

  8. MRVP broth IMViC Series Voges-Proskauer Test Leave uncapped for 15-20 minutes……..

  9. + - IMViC Series Citrate Utilization Test

  10. Slow positive Urease Test Positive result (alkaline) Negative result (acidic) Uninoculated urea agar

  11. Motility Test

  12. A B • Answers: • A 3. B • 2. A 4. B

  13. What is the name of the lab procedure demonstrated here? Answer: Stabbing the butt!

  14. Use the chart on p. 181 and or the flow chart on p. 182 of your lab manual to figure out who I am! U I M V C Answer: Shigella sonnei

  15. Use the chart on p. 181 and/or the flow chart on p. 182 of your lab manual to figure out who I am! U I M V C Answer: Escherichia coli

  16. Use the chart on p. 181 and/or the flow chart on p. 182 of your lab manual to figure out who I am! U M C V I Answer: Serratia marcesens

  17. Use the chart on p. 181 and/or the flow chart on p. 182 of your lab manual to figure out who I am! I M C V Answer: Klebsiella pneumoniae

  18. Use the chart on p. 181 and/or the flow chart on p. 182 of your lab manual to figure out who I am! U I M C V Answer: Salmonella typhimurium

  19. Use the chart on p. 181 and/or the flow chart on p. 182 of your lab manual to figure out who I am! U I C M V Answer: Proteus vulgaris

  20. Use the chart on p. 181 and/or the flow chart on p. 182 of your lab manual to figure out who I am! U I M C V Answer: Enterobacter aerogenes

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