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Experience with Roche´s COBAS AmpliPrep/COBAS TaqMan96 automated PCR system in two large blood bank settings

Lutz Pichl , DRK-Blutspendedienst West Christine Jork, DRK-Blutspendedienst NSTOB. Experience with Roche´s COBAS AmpliPrep/COBAS TaqMan96 automated PCR system in two large blood bank settings . Christine Jork, DRK-Blutspendedienst NSTOB Lutz Pichl , DRK-Blutspendedienst West. Springe.

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Experience with Roche´s COBAS AmpliPrep/COBAS TaqMan96 automated PCR system in two large blood bank settings

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  1. Lutz Pichl , DRK-Blutspendedienst West Christine Jork, DRK-Blutspendedienst NSTOB Experience with Roche´s COBAS AmpliPrep/COBAS TaqMan96 automated PCR system in two large blood bank settings Christine Jork, DRK-Blutspendedienst NSTOB Lutz Pichl , DRK-Blutspendedienst West

  2. Springe DRK-BSD West DRK-BSD NSTOB Hagen

  3. System configuration

  4. 842,751(07/2005 -05/2006) HCV: Ø 0.27 %HIV-1: Ø 0.12 %HBV: Ø 0.05 % HCV: Ø 1.68 %HIV-1: Ø 0.15 %HBV: Ø 0.14 % HCV: 2 (05) 2 (06)HBV: 1 HIV-1: 2 (06)HBV: 1 (06)

  5. CAP/CTM Maintenance Sample Preparation Result Validation Data Transfer (LIMS) NA Extraction Amplification & Detection Pooling HIV-1 3h 15‘ 15-30‘ 0,5 h 1 h 2 h HCV 2h 40‘ HBV 2h 10‘ HAV/PVB 50‘ ~1 p.m. 6 a.m. Turn around time

  6. Pros & Cons of CAP/CTM96 System

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