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PLASMIDS, BY CHARLOTTE, SUSAN, IDI AND AMANDA

PLASMIDS, BY CHARLOTTE, SUSAN, IDI AND AMANDA. . Charlotte. PAP. SEARRF. PAP. 6H. V5. PAP. MUT. PAP. For Cloning into PET. Susan. PAP. SEARRF. PAP. PAP. PAP. 6H. V5. DEL. MUTATION. Forward: Translate DNA Sequence Untitled Seq #33(1,590 ) With Standard Genetic Code 

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PLASMIDS, BY CHARLOTTE, SUSAN, IDI AND AMANDA

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  1. PLASMIDS, BY CHARLOTTE, SUSAN, IDI AND AMANDA

  2. Charlotte PAP SEARRF PAP 6H V5 PAP MUT PAP For Cloning into PET Susan PAP SEARRF PAP PAP PAP 6H V5 DEL

  3. MUTATION Forward: • Translate DNA Sequence Untitled Seq #33(1,590) With Standard Genetic Code  • Molecular Weight 20303.75 Daltons • 176 Amino Acids • 24 Strongly Basic(+) Amino Acids (K,R) • 16 Strongly Acidic(-) Amino Acids (D,E) • 47 Hydrophobic Amino Acids (A,I,L,F,W,V) • 39 Polar Amino Acids (N,C,Q,S,T,Y) Reverse • Translate DNA Sequence Untitled Seq #42(1,307) • With Standard Genetic Code • Molecular Weight 11053.48 Daltons • 95 Amino Acids • 23 Strongly Basic(+) Amino Acids (K,R) • 8 Strongly Acidic(-) Amino Acids (D,E) • 18 Hydrophobic Amino Acids (A,I,L,F,W,V) • 25 Polar Amino Acids (N,C,Q,S,T,Y)

  4. DELETION Translate DNA Sequence 1R68G Fwd Primer.seq(1,562) • Molecular Weight 21076.21 Daltons • 187 Amino Acids • REVERSE PRIMER • Translate DNA Sequence Untitled Seq #39(1,286) • Molecular Weight 11001.39 Daltons • 95 Amino Acids

  5. Idiatu CT-PAP NT-PAP SEARRF 6H V5 CT-PAP NT-PAP 6H V5 SEARRF Into pET151 Amanda PAP SEARRF PAP 6H V5 PAP PAP 6H V5 MUT Into pET151

  6. R68 MUTATION WITH THE INTACT AND MUTATED ACTIVE SITES

  7. PAP WITHOUT CT: INTACT AND MUTATED R68

  8. PCR PRODUCT FROM THE MINI PREP

  9. IdI PCR

  10. Now we have some questions for you! • 

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