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Peonies and Viruses Indexing

Peonies and Viruses Indexing. Nancy L. Robertson Research Plant Pathologist USDA-ARS Arctic & Subarctic Plant Gene Bank Palmer, Alaska. Alaska Peony Growers Conference Jan. 27-28, 2011. VIRUSES & PEONIES. Alfalfa mosaic virus 2003 Italy Cucumber mosaic virus 2010 France

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Peonies and Viruses Indexing

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  1. Peonies and VirusesIndexing Nancy L. Robertson Research Plant Pathologist USDA-ARS Arctic & Subarctic Plant Gene Bank Palmer, Alaska Alaska Peony Growers Conference Jan. 27-28, 2011

  2. VIRUSES & PEONIES • Alfalfa mosaic virus • 2003 Italy • Cucumber mosaic virus • 2010 France • Tomato spotted wilt virus • ????? USA • Tobacco rattle virus • 1976 Japan • Raspberry ringspot virus • 1985 Finland • Strawberry latent ringspot virus • 1985 Finland

  3. Geographical Confirmation of Tobacco rattle virus in Peony • Japan 1976 • Cheng et al. • New Zealand 1978 • Jones & Young • China 1990 • Ningshen & Yunfeng • Lithuania 2000 • Samuitiene & Navalinskiene • Alaska 2009 • Robertson et al.

  4. Peony Plots 2007

  5. Symptoms: ringspots on leaves

  6. Tobacco rattle virus isolatefrom peony RT-Polymerase chain reaction assay Genomic Sequence of Virus for Definitive Identification • Wide Plant Host Range > 400 Species

  7. TRV NEMATODE VECTORParatrichodorus minor Nematode feeding on root hair University of Florida

  8. Virus Diagnostics LEAF MATERIAL SAP INOCULATIONS HOST RANGE TOTAL NUCLEIC/ TOTAL RNA MINIPURIFICATIONS PCR PROTEIN SEQUENCING GENOME RNA ELECTRON MICROSCOPY ELISA WESTERN SEQUENCING

  9. Control Viruses • Obtain Certified Virus-Free Stocks • Maintain Healthy Stocks • Visual Inspection • Elimination of Affected Plant • Control Insect & Nematode Vectors

  10. Virus Elimination Heat Therapy of Affected Plant Eliminates Viruses Meristem Tip Culture Cryotherapy/Thermotherapy Propagation of Virus-Free Peony Seedlings

  11. THANKS TO COLLABORATORS Dr. Patricia Holloway Dr. Loretta Winton Gail Wright (STUDENT WORKER)

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