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Data from Kamchatka 2004

Data from Kamchatka 2004. Elizabeth A. Burgess. eab. Arkashin Shaft. Estimated numbers of sulfate-reducers per 0.5 mL. In situ: T=60 o C, pH=5, [SO 4 2- ]=82 mg/L. AN04-015D – DNA from sediment Arsenate-reducer enrichments in glycerol stock. eab. Orange Field.

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Data from Kamchatka 2004

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  1. Data from Kamchatka 2004 Elizabeth A. Burgess eab

  2. Arkashin Shaft Estimated numbers of sulfate-reducers per 0.5 mL In situ: T=60oC, pH=5, [SO42-]=82 mg/L AN04-015D – DNA from sediment Arsenate-reducer enrichments in glycerol stock eab

  3. Orange Field Estimated numbers of sulfate-reducers per 0.5 mL In situ: T=99oC, pH=7, [SO42-] not available AN04-008D, 009D, 010D – DNA from core samples? eab

  4. Sunshine Swamp Estimated numbers of sulfate-reducers per 0.5 mL In situ: T=63oC, pH=6, [SO42-]=148 mg/L AN04-013D, 014D – DNA from core samples eab

  5. Yellow-Blue Estimated numbers of sulfate-reducers per 0.5 mL In situ: T=57oC, pH=6, [SO42-]=55 mg/L AN04-001D to 007D, 017D – DNA from core and mat Sulfate-reducer enrichments in glycerol stock eab

  6. Jenn’s Pools Estimated numbers of sulfate-reducers per 0.5 mL In situ: V1: T=80oC, pH=5, [SO42-]=188 mg/L V2: T=88oC, pH=6, [SO42-]=124 mg/L N: T=77oC, pH=4, [SO42-] not avail E: T=77oC, pH=6, [SO42-] not avail eab

  7. Central Thermal Field Estimated numbers of sulfate-reducers per 0.5 mL In situ: BG: T=77oC, pH=6, [SO42-] not avail OG: T=60oC, pH=4, [SO42-] not avail eab

  8. Bay of Pippe Estimated numbers of sulfate-reducers per 0.5 mL In situ: BG: T=77oC, pH=6, [SO42-] not avail OG: T=60oC, pH=4, [SO42-] not avail eab

  9. Estimated Numbers of Sulfate-Reducing Prokaryotes by Site eab

  10. Pending Data Arkashin and Yellow-Blue: - Reduction Rate Assays - Culturing and Isolation - 16S Clone Libraries eab

  11. Experiments for 2005 Reduction Rate Assays Enumerations of Arsenate-Reducers Culturing Techniques eab

  12. Yellow-Blue (con’t) Preliminary TRFLP results Archaeal primer set Bacterial primer set one enzyme along gradient top  bottom two enzymes along gradient top  bottom No visible products negative controls Low diversity and similarity may be artifacts of analysis…

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