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Microscopes

Microscopes. Compound Bright-Field Light Microscope Maximum Useful Magnification = 1200X Why?. Resolution. Useful magnification is limited by Resolution Resolution is influenced by Numerical Aperture (NA) NA determined by Wavelength of Light and Refractive Index Improve Magnification

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Microscopes

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  1. Microscopes • Compound Bright-Field Light Microscope • Maximum Useful Magnification = 1200X • Why?

  2. Resolution • Useful magnification is limited by Resolution • Resolution is influenced by Numerical Aperture (NA) • NA determined by Wavelength of Light and Refractive Index • Improve Magnification Shorten Wavelength Increase Refractive Index

  3. Staining • Simple Stains • Light Microscope - Colored Dye • Electron Microscope - Electron Dense • Differential Stains Gram Stain

  4. Gram Stain Most Important Stain Key Bacteriological Test Conventional Stain Multiple Steps & Subjective Fluorescent Stain One Step & +/-

  5. Optical Tricks • Oil Immersion Lens • Dark Field Microscope

  6. Other Microscopes Phase Contrast Microscopes

  7. Fluorescent Microscopes

  8. More Microscopes • Interference Contrast Microscope • Atomic Force Microscope

  9. Confocal Scanning Laser Microscope

  10. Electron Microscopes Shorten wavelength • Scanning Electron Microscope- SEM 3 Dimensional images

  11. Transmission Electron Microscope • TEM • Better Resolution

  12. Microscopes Word List

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