1 / 15

Gram-negative rods: Enterobacteriaceae Part III

Gram-negative rods: Enterobacteriaceae Part III. Karen Honeycutt, M.Ed., MT(ASCP)SM MT 418 Clinical Microbiology Student Laboratory Session. GNR: Enterobacteriaceae. Isolation. Growth on BAP at 24 hrs, ambient air, CO 2 or anaerobically (facultative anaerobe)

zarola
Download Presentation

Gram-negative rods: Enterobacteriaceae Part III

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Gram-negative rods:Enterobacteriaceae Part III Karen Honeycutt, M.Ed., MT(ASCP)SM MT 418 Clinical Microbiology Student Laboratory Session Microbiology

  2. GNR: Enterobacteriaceae Isolation • Growth on BAP at 24 hrs, ambient air, CO2 or anaerobically (facultative anaerobe) • In general colony morphology: large, gray, beta- or nonhemolytic(normally larger than Staph, Strep, & Enterococci) Microbiology

  3. GNR: Enterobacteriaceae Characteristics Utilization of glucose (OF Glucose): Microbiology

  4. GNR: Enterobacteriaceae Characteristics Oxidase reaction: Microbiology

  5. GNR: Enterobacteriaceae Characteristics Growth on MAC: Yes Microbiology

  6. GNR: Enterobacteriaceae Characteristics Nitrate reduction: NO3 to NO2 Microbiology

  7. GNR: Enterobacteriaceae Characteristics Flagellar arrangement: peritrichous Microbiology

  8. GNR: Enterobacteriaceae Biochemicals – ONPG Rapid Lactose “F” requires permease enzyme ONPG similar to lactose, doesn’t require permease enzyme to enter the organism ONPG + beta-galactosidease = yellow color Slow lactose fermenter (appear as NLF on MAC, KIA, etc.) and Rapid lactose fermenter (appear as LF on MAC & KIA are POSITIVE Microbiology

  9. GNR: Enterobacteriaceae Nitrate to Nitrite to Nitrogen Gas Inoculate to media with nitrate Incubate test 18-24 hrs Add reagent Reagent reacts with nitrite to form red color. This reaction indicates: What did the work? NO3 to NO2 Organism Microbiology

  10. GNR: Enterobacteriaceae Biochemicals – Nitrate After nitrate rgt addition: Zinc will reduce nitrate to nitrite Add zinc dust What did the work? Zinc Therefore, the organism is: Nitrate negative Microbiology

  11. GNR: Enterobacteriaceae Biochemicals – Nitrate After nitrate rgt addition: Zinc will reduce nitrate to nitrite Add zinc dust What did the work? Organism reduced nitrate to nitrogen gas Therefore, the organism is: Nitrate positive Microbiology

  12. GNR: Enterobacteriaceae Identification – Categories Lactose “F” Lysine, phenyalalanine or tryptophane deaminase positive? Lactose NF & LDA/TDA/PDA negative Microbiology

  13. ODC LIA KIA Indole GNR: Enterobacteriaceae Identification Motile HE Identification = Salmonella sp. Microbiology

  14. ODC LIA Citrate KIA Indole GNR: Enterobacteriaceae Identification Motile Identification = Escherichia coli Microbiology

  15. MacConkey ODC KIA LIA Urea Indole GNR: Enterobacteriaceae Identification Identification = Proteus vulgaris Microbiology

More Related