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S- Sepharose C-101

S- Sepharose C-101. Andy Hunter Alec Mackenzie Trevor Seelert. Purpose of Unit. Separate Proinsulin and other proteins from basic impurities Prepare the stream for further downstream purification. Cation exchange Chromatography. Equilibrate Load Rinse Elute Wash Regenerate.

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S- Sepharose C-101

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  1. S-SepharoseC-101 Andy Hunter Alec Mackenzie Trevor Seelert

  2. Purpose of Unit • Separate Proinsulin and other proteins from basic impurities • Prepare the stream for further downstream purification

  3. Cation exchange Chromatography • Equilibrate • Load • Rinse • Elute • Wash • Regenerate

  4. Process Flow Sheet

  5. Process Flow Sheet New Design

  6. Process Flow Sheet New Design

  7. S-Sepharose Design • Scale-up keeping constant values

  8. S-Sepharose Design • Scaled-up from US 101013 Patent

  9. S-Sepharose Design • GE Chromaflow • GE AxiChrom

  10. S-Sepharose Design • GE Chromaflow Column • Currently max D = 1200mm, H = 30cm • Custom Design available for D > 1200mm

  11. Economics and Suppliers • S-Sepharose available from GE Healthcare • Chromaflow supplied by GE

  12. IEC Alternatives • SP-Sepharose: Sulphopropyl • –O–CH2CHOHCH2OCH2CH2CH2SO3– • S-Sepharose: Sulphoethyl • no longer produced • –O–CH2–CH2–SO3– • Replaced by SP-Sepharose • Anion exchange resin • Q-sepharose • DEAE • HIC Columns • SEC/Gel Filtration

  13. IEC Alternatives • Comparison of IEC

  14. No Questions Allowed Disclaimer: The views presented in this slide are not necessarily those of any or all team members.

  15. Just Kidding!

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