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Design of an integrated liquid flow cell for correlative microscopy

Design of an integrated liquid flow cell for correlative microscopy. Diederik Morsink 15-8-2013 First Msc. presentation. Outline. Crash course in Life science imaging Introducing Delmic Previous research My Project Progress To do Aimed results Planning.

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Design of an integrated liquid flow cell for correlative microscopy

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  1. Design of an integrated liquid flow cell for correlative microscopy Diederik Morsink 15-8-2013 First Msc. presentation

  2. Outline • Crash course in Life science imaging • Introducing Delmic • Previous research • My Project • Progress • To do • Aimed results • Planning

  3. Crash course in Life science imaging • Highest goal in life science imaging: High resolution imaging of living cells in their natural environment ~1675 2013 http://www.blatner.com/adam/consctransf/historyofmedicine/1-overview/brief2.html http://www.ceo.msu.edu/news.html

  4. Crash course in Life science imaging • Fluorescence Microscopy • Scanning Electron Microscopy • Correlative Microscopy

  5. Crash course in Life science imaging Fluorescence microscopy • Advantages: • Color contrast • No damage to sample • Functional information • Disadvantage: • Diffraction limited http://www.piercenet.com/

  6. Crash course in Life science imaging Fluorescence microscopy • Advantages: • Color contrast • No damage to sample • Functional information • Disadvantage: • Diffraction limited Image courtesy: Nalan Liv

  7. Crash course in Life science imaging Scanning electron microscopy (SEM) • Advantage: • High resolution • Structural information • Disadvantage: • Needs vacuum environment • Can damage the sample http://www.ammrf.org.au/myscope/sem/background/

  8. Crash course in Life science imaging Scanning electron microscopy (SEM) • Advantage: • High resolution • Structural information • Disadvantage: • Needs vacuum environment • Can damage the sample Image courtesy: Nalan Liv

  9. Crash course in Life science imaging Correlative Light and Electron Microscopy (CLEM) • Combining the information of from SEM and Fluorescence imaging • Structural and functional information

  10. Crash course in Life science imaging Correlative Light and Electron Microscopy (CLEM) • Combining the information of from SEM and Fluorescence imaging • Structural and functional information 3μm Image courtesy: Nalan Liv

  11. Delmic • Spinoff TNW • SECOM platform • Correlative microscopy Image courtesy: Delmic and Ruud van Tol

  12. Previous research • Liquid cell • Liquid flow cell

  13. Previous research • Liquid cell • Liquid Flow cell Image courtesy: Daan van Oosten Slingeland

  14. Previous research • Liquid cell • Liquid Flow cell Image courtesy: Daan van Oosten Slingeland

  15. My project .... Liquid cell Liquid Flow cell Image courtesy: Daan van Oosten Slingeland

  16. My project • Integrating pump, imaging area and reservoirs in a single MEMS device. • Simultaneous imaging and manipulation • Living cells • Working prototype

  17. Progress • So Far: • Literature study on Life science imaging techniques • Literature study on Micropumps • Requirements • Selecting micropump designs • Conceptual design of how to integrate pump, imaging area and reservoirs in a single device

  18. Requirements

  19. Conceptual design:

  20. Conceptual design:Microfluidic pump • Based on designs by Linnemann (1998) and Kang (2008) • Stack of 3 Si wafers pumpchamber Pump diaphragm Piezo element Inlet Outlet

  21. Conceptual design:Imaging area • Separate chip with silicon nitride membrane (orange) Silicon nitride membrane Imaging area Silicon spacer Microchannel Glass coverslip

  22. Conceptual design:Integration in a single device

  23. Conceptual design:Integration in a single device

  24. Conceptual design:Integration in a single device

  25. Conceptual design:Integration in a single device Outlet Inlet

  26. Conceptual design:Integration in a single device Al. top O-ring Si wafer 3 Sii wafer 2 Si3N4 Chip Si wafer 1 Coverslip O-ring Al. bottom

  27. Conceptual design:Integration in a single device Al. top O-ring Si wafer 3 Sii wafer 2 Si3N4 Chip Si wafer 1 Coverslip O-ring Al. bottom

  28. Design overview • Two microfluidic pumps and reservoirs • Imaging area with separate silicon nitride chip • Stack of 3 silicon wafers on glass substrate

  29. Fabrication

  30. Fabrication • Manufacturing complete device not feasible (time restriction)

  31. Fabrication • Manufacturing complete device not feasible (time restriction) • Two critical steps: 1. Bonding coverslip-silicon 2. Clamping chip with silicon nitride membrane F F 2. 1.

  32. To do • Gain experience with bonding coverslip-silicon techniques for this purpose • Build a simple microfluidic device that demonstrates that the chosen bonding technique is appropriate

  33. To do • Gain experience with bonding coverslip-silicon techniques for this purpose • Build a simple microfluidic device that demonstrates that the chosen bonding technique is appropriate • If time permits: • Develop a suitable way for clamping silicon nitride chip to silicon spacer • Build a simple microfluidic device that demonstrates successful clamping of silicon nitride chip

  34. Final results aimed for • Conceptual design for an integrated liquid flow cell for correlative microscopy • Simple microfluidic device demonstrating successful manufacturing of a critical step in the process

  35. Planning

  36. Design of an integrated liquid flow cell for correlative microscopy Diederik Morsink First Msc. presentation

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