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Multi-site recordings in Tg4510 mice

Multi-site recordings in Tg4510 mice. Liam Scott. Hippocampus: Area. Area (mm2). 3m. 7m. 7 month male Tg4510 mice show decreased MWM performance and hippocampal cell loss. 3 vs. 7 month old Tg4510 male mice show:. (Wildtype for comparison). Data: Kawabe, Klein, Milici.

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Multi-site recordings in Tg4510 mice

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  1. Multi-site recordings in Tg4510 mice Liam Scott

  2. Hippocampus: Area Area (mm2) 3m 7m 7 month male Tg4510 mice show decreased MWM performance and hippocampal cell loss 3 vs. 7 month old Tg4510 male mice show: (Wildtype for comparison) Data: Kawabe, Klein, Milici Data: Yabe, Moore, Roof

  3. EEG Stimulus Hippo Theta Frequency NPO 8.0 • Medial Septum • Hippocampal Formation 7.5 • pcells 7.0 Frequency (Hz) 6.5 • Glu • Glu • Glu • gaba • gaba • gaba • gaba • gaba • ACh 6.0 5.5 0.06 0.08 0.10 0.12 0.14 0.16 Stim Current (mA) Theta Power 50.0k • Supramammilary Bodies • (SUM) 45.0k 40.0k Theta Power (V2) • 5-HT • 5-HT 35.0k 30.0k • = + • = - 25.0k • Nucleus Pontis Oralis • (nPO) • Median Raphe 20.0k 15.0k • Adapted from: RP Vertes (2005) Hippocampus 15:923-35 0.06 0.08 0.10 0.12 0.14 0.16 Stim Current (mA) Stimulation of the nPO drives the hippocampal EEG in anesthetized rats and mice 6-seconds

  4. Determining electrode position using stimulation of nPO

  5. 7 month male Tg4510 mice show decreased nPO-evoked hippocampal theta power and total power Relative Theta Power Peak Frequency Theta Power Total Power * * * * * * * * ** * * * * ** * ** * * ** * (*=P<0.05, **=P<0.01, ***=P<0.001 ; unpaired t-test)

  6. Perforant path – dentate gyrus LTP

  7. Determining electrode position using stimulation of perforant path

  8. 7 month male Tg4510 mice show reduced amplitude of perforant path-evoked dentate gyrus population spike

  9. 7 month male Tg4510 mice show reduced amplitude of perforant path-evoked dentate gyrus population spike

  10. 7 month male Tg4510 mice show no change in perforant path - dentate gyrus LTP

  11. control CA-1 DG

  12. Tg CA-1 CA1 population spike DG DG population spike

  13. Summary Stimulated theta • No change in theta frequency or relative theta • Reduction in theta power and total power PP-DG LTP • Reduction in amplitude of evoked population spike • No change in LTP due to HFS All observations can be explained by reduction in cell number in hippocampal formation???

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