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Tritiated (3H) thymidine

3 H-thymidine. Tritiated (3H) thymidine. 3 H-thymidine. Original method for measuring cell proliferation Radioactive Not compatible for multiplexed analyses. Br. Br. Br. Br. BrdU. BrdU (5-bromo-2 ' -deoxyuridine). Br. Br. Br. Br. BrdU. Acid or DNase. Br. Br. Br. Br. BrdU.

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Tritiated (3H) thymidine

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  1. 3H-thymidine Tritiated (3H) thymidine

  2. 3H-thymidine • Original method for measuring cell proliferation • Radioactive • Not compatible for multiplexed analyses

  3. Br Br Br Br BrdU BrdU (5-bromo-2'-deoxyuridine)

  4. Br Br Br Br BrdU

  5. Acid or DNase Br Br Br Br BrdU

  6. Br Br Br Br BrdU

  7. Br Br Br Br BrdU • Non-radioactive • Multiplex compatible but, strand separation requirement for anti-BrdU access, can affect: • Ability for other antibodies to bind • Morphology • Ability for dyes that require dsDNA to bind efficiently, i.e., cell cycle dyes

  8. Click-iT™ EdU EdU (5-ethynyl-2'-deoxyuridine)

  9. Click-iT™ EdU

  10. Click-iT™ Edu • Non-radioactive • No DNA denaturation required • Simplified protocol • Small molecule detection • Multiplex compatible, including • Other antibodies • Dyes for cell cycle analysis

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