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Mycorrhiza activity in Cypripedium calceolus roots

17-19 May 2013, Rende , Italy. Mycorrhiza activity in Cypripedium calceolus roots. Ieva Druva-Lūsīte , Dace Kļaviņa National Botanic Garden , Latvia. C ypripedium c alceolus is included in R ed D ata B ook of Latvia as vulnerable species and listed on Annexes II and IV

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Mycorrhiza activity in Cypripedium calceolus roots

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  1. 17-19 May 2013, Rende, Italy Mycorrhiza activity in Cypripedium calceolus roots IevaDruva-Lūsīte, Dace Kļaviņa NationalBotanicGarden, Latvia

  2. Cypripediumcalceolusis included in RedDataBookof Latvia as vulnerable species and listed on Annexes II and IV of the Habitats Directive and Appendix II of the Berne Convention.

  3. Backgroundofstudy Imature seeds Seedling Mycorrhization Seedling after cold storage Second season after ex vitro Seedlings with first leaves

  4. TheObjective To evaluate the seasonal dynamic of mycorrhiza activity in C.calceolus roots in natural habitats and in NationalBotanicGarden area.

  5. SpreadofC.calceolusandsamplingplaces 1. Sloka 2. Ķemeri 3. NBG 4. Silzemnieki

  6. PopulationofC. calceolusinallterritoryofLatvia Numbers

  7. Largeclampsandjuvenilesinsampligplaces %

  8. Sloka pHHCl 5.1 Organicmatter: 37% Vitalityofpopulation: poor Habitat: mixedbroad-leaved - coniferousforest

  9. Ķemeri pHHCl7.1 Organicmatter: 20% Vitalityofpopulation: good Habitat: broad-leaveddeciduousforest

  10. Silzemnieki pHHCl6.4 Organicmatter: 19% Vitalityofpopulation: excellent Habitat: broad-leaveddeciduousforest

  11. ArboretumofNationalBotanicGarden pHHCl6.3 3-4 yearsoldseedlings Habitat: arboretumwithCorylusavelana

  12. Methodofmycorrhizaestimation • Mycorrhiza Manual 2001, www2.dijon.inra.fr/mychintec/Protocole/protoframe.html • Valuesput into thecomputer program “Mycocalc” to calculate M% - intensityoractivity of the mycorrhizalcolonisation in the root system. • N=50 eyeshotsinonerepeat, minimum – threerepeats. • Rootsclearedin 10% KOH for 1h; roots rinsed with water; stainedin 0.05% trypanbluefor 5 min; storedinlactoglicerol.

  13. Mycorrhizaactivityin Sloka M%

  14. Mycorrhizaactivityin Ķemeri M%

  15. MycorrhizaactivityinSilzemnieki M%

  16. Mycorrhizaactivityinarboretumof NBG M%

  17. MycorrhizaactivityindormantCypripediumcalceolusin Ķemeri M%

  18. Conclusions • Mycorrhizaactivitywashigherinthebeginingofvegetationseasoningoodandexcellentorchidpopulations. Then it slowly decreased till August. • MycorrhizaactivityshowedstabilityofhabitatandorchidpopulationinSilzemnieki. • Inaveragemycorrhizaactivityin Sloka was a comparativelyhigherthaninarboretumofNationalBotanicGarden. • Nextstep – to investigatemycorrhizaspeciesinC.calceolusroots.

  19. Cypripediumcalceolus 200x 400x 200x

  20. Acknowledgements Thisstudywasfundedby LatvianEnvironmentalProtectionFund. SoilchemicalanalysesmadeatLaboratory of Plant Mineral Nutrition,InstituteofBiology, UniversityofLatvia. Thanksforpermission to usemicroscope to LaboratoryofMycologyandPlantPathology, Latvian State Forest Research Institute "Silava“.

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