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Sysmex XE-2100

Sysmex XE-2100. Reticulocyte Detection. Reticulocyte Methodology. semiconductor laser flow cytometry method. RET-SEARCH (II) (diluent) RET-SEARCH (II) dye (stain) passed through the laser flow cytometer for analysis . Counted using forward light scatter and side fluorescence.

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Sysmex XE-2100

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  1. Sysmex XE-2100 Reticulocyte Detection

  2. Reticulocyte Methodology • semiconductor laser flow cytometry method. • RET-SEARCH (II) (diluent) • RET-SEARCH (II) dye (stain) • passed through the laser flow cytometer for analysis. • Counted using forward light scatter and side fluorescence.

  3. Flow Cytometry • Forward light scatter - size. • Side fluorescence - nuclear content.

  4. RETIC Channel Explained

  5. Therefore the RETIC Scattergram …

  6. RETIC “Differential” • Retic RNA content decreases on maturation. • Thus fluorescence decreases with maturation. • The XE2100 resolves the differential stages by fluorescence intensity.

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