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Safety in a Bioengineering Lab

Safety in a Bioengineering Lab. Safety rule #1. Sure, you are safe…. Even if you think you are not going to have a problem (and you don’t need goggles, etc).…. Safety rule # 2: Be cautious and vigilant the person next to you may not be following safety rules.

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Safety in a Bioengineering Lab

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  1. Safety in a Bioengineering Lab

  2. Safety rule #1

  3. Sure, you are safe…. • Even if you think you are not going to have a problem • (and you don’t need goggles, etc).…. • Safety rule # 2: Be cautious and vigilant • the person next to you may not be following safety rules

  4. Safety Rule #3Dress for the lab No! Yes! Hair pulled back Sleeves can be rolled up Long pants in sturdy fabric Closed toe shoes Minimal jewelry

  5. Dressed for the lab on runways

  6. Safety Rule #4— Chemicals can be hazardous! • Know the hazards of chemicals you use • Caustic—burns the skin or eyes • Volatile—evaporates and fumes can be inhaled • Flammable—can catch on fire easily • Toxic orcarcinogenic—can cause illness or death in short term or long term • Listen to your instructor • READ LABELS and remain focused

  7. Safety Rule # 5-Be careful around sharp objects Scalpels, razor blades, broken glass Small cuts are #1 injury in lab classes • Dispose all used sharp items into a sharps container • Razor blades • Any one time use glassware • Broken glassware will be taken care of by helpers

  8. Safety rule #6 Avoid burns on hot items #7- Use caution around electricity • Burns caused by retrieving sample from boiling water is the #2 injury in classes. • Watch for water near electric appliances.

  9. Safety rule #8- Clean up after yourself • Reduce clutter • Improve visibility • Work efficiently • Keep yourself and others safe

  10. Proper clean-up of biological materials Biological samples will degrade • Degrading organisms attract bacteria, mold • will ruin future experiments • a biohazard • smell

  11. Safety rule #9- No food or drinks in the culture room! • NO drinks, gum, mints, make-up. • Saliva and crumbs from you can get on your work samples • Contaminate your work • Your work samples or their by-products can get on your food! • And then into your mouth!!

  12. “Invisible” life forms are everywhere! • Present on skin, fall off of hair, clothing, in saliva, tears, breath. • Present on surfaces and in water • Especially warm water

  13. Safety rule #10- Protecting your cultures protects you! • Biosafety hood (Class II safety cabinet)

  14. Aseptic Technique • Prevents introduction of organisms into your cultures • Roll sleeves up, pull hair back, remove rings, watches, bracelets • Wash your hands • 70% ethanol • A spray-on hand sanitizer • Gloves by suggestion • Wash hands afterwards, gloves or not

  15. Safety rule #11- Proper biological disposal is absolutely essential! • For cell cultures (Biosafety level 1) • Hazardous to dispose of any LIVING organism by dumping in trash or rinsing down the sink • Bleach will kill any unused cells • Squirt small amount into media and cells • Wait a few minutes • Rinse down sink with plenty of running water

  16. Proper disposal of biological waste • Bacterial and fungal cultures • Autoclave • Use autoclave bags

  17. Other hazards in a bioengineering laboratory • Compressed gases. • Wear goggles when using compressed gas • Cryogenic liquids • To preserve cells • Can cause “burns” What is wrong with this picture?

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