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PFK through the ages

PFK through the ages. Jonathan A. Atwater Janet A. Baker Chitra Chandrasekaran Lizette Santos-Santori. Project goal.

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PFK through the ages

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  1. PFK through the ages Jonathan A. Atwater Janet A. Baker Chitra Chandrasekaran Lizette Santos-Santori

  2. Project goal • The primary goal of this project is to put metabolism in an evolutionary perspective by analyzing the similarities and differences among taxonomic groups for ATP-dependent phosphofructokinase.

  3. Project audience • The project will be directed to undergraduate students in the following courses: • General Biology • Cell Biology • Biochemistry

  4. Clostridium tetani Bacillus cereus Anabaena variabilis Bateriodes fragilis Thermus aquaticus Uncultured archaea Thermoproteus tenax Arabadopsis thaliana Sacchromyces cerevisiae Mus musculus Homo sapiens Pan troglodytes Xenopus laevis Danio rerio Drosophila melanogaster Caenorhabditis elegans Project materials • Data base file of amino acid sequences for phosphofructokinase for the following organisms:

  5. General Objectives • Problem-based approach to examining protein structure and function from an evolutionary perspective • Using the evolutionary perspective to provide a unified approach to link the study of protein structure and function to metabolism

  6. Specific objectives • Analyze amino acid sequence for each organism. • Perform a multiple sequence alignment: CLUSTALW. • Use the alignment to identify important functional domains. • Infer phylogenetic relationships.

  7. Future plans • Include more organisms in the study. • Use amino acid sequence alignment to examine protein structure at a three dimensional level (Protein Explorer). • Examine the evolution of active sites and allosteric regulatory sites of phosphofructokinase • Establish evolutionary connections by phylogenetic analyses.

  8. Resources/references • www.ncbi.nlm.nih.gov (NCBI) • www.bioquest.org/bedrock (BioQuest) • http://workbench.sdsc.edu (Biology Workbench) • http://molvis.sdsc.edu/protexpl/frntdoor.htm (Protein Explorer)

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