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La scienza delle misure chimiche e qualità della vita Mario Gallorini

La scienza delle misure chimiche e qualità della vita Mario Gallorini I.N.R.I.M Unità di Radiochimica e Spettroscopia c/o Università degli Studi di Pavia. Torino - 25 Gennaio 2007. INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia.

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La scienza delle misure chimiche e qualità della vita Mario Gallorini

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  1. La scienza delle misure chimiche e qualità della vita Mario Gallorini I.N.R.I.M Unità di Radiochimica e Spettroscopia c/o Università degli Studi di Pavia Torino - 25 Gennaio 2007

  2. INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

  3. INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

  4. INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

  5. INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

  6. EUROTERVIT - JRC- Ispra1990-2000 INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

  7. INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

  8. INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

  9. INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

  10. INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

  11. TRACE METALS DETERMINATION IN BIOLOGICAL SPECIMENS SAMPLING Trace metals released from surgical Procedure used to evaluate possible TM contamination tools in collecting rabbit lung tissues. during samples collection Radio - release procedure. Characterization and Element bistoury forcep human Identification of the analysis of the materials A (ng) (ng) lung w.t. potential contaminants w hich come in contact with the samples (ref. Value ng/g) Ag Neutron activation of the Production of the materials (bistoury, corresponding B needles, forceps contaminants radionuclides Contact of the irrad iate materials with the Identification of the C samples contaminats (radio - release method) NAA of “uncontamined” sample in comparison D Quantification of the with the same sample degree of contamination treated with the sampling materials 1.3 120 2.1 Ce 25 80 87 Co 3.7 25 80 Cr 50 3200 312 Cs 0.7 1.3 17.2 Fe 170 0 30000 2640000 Hg < 10 17 90 Ir 0.02 0.02 0.2 Ni 135 450 600 Rb 37 140 4775 Sb 22 35 230 Ta 2 14 13 Zn <30 120 12130 INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

  12. INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

  13. INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

  14. INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

  15. INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

  16. INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

  17. INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

  18. INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

  19. INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

  20. INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

  21. INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

  22. The application of the Neutron Activation Analysis for trace metals determination in human brain tissues and substantia nigra neuromelanin In human brain many transition metals are necessary for important biological processes, and alterations in their homeostasis can produce neurotoxic effects. Toxicity pathways frequently involve oxidative stress through an increase of free radicals production (redox- reactions). The subsequent damages or death of cells can lead to neurodegenerative diseases, such as Parkinson’s Disease (PD) and Alzheimer Disease (AD) INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

  23. Trace metals and human health – Parkinson disease Trace elements analysis in human brain tissues is an important step in understanding the PD pathogenesis. Thanks to a controlled separation procedure, NM was isolated from SN with no metal contamination. NM showed a great ability to bind large amounts of metals, especially Fe, suggesting that NM can play a protective role for brain neurons against the free toxic radicals deriving from redox active metal ions. Fe and Cu concentrations in brain tissues increase along with aging in normal subjects. INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

  24. INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

  25. Human substantia nigra in parkinsonian (A) and in normal subject (B) A B INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

  26. Substantia nigra pars compacta of normal (A) and parkinsonian subjects (B) A B INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

  27. INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

  28. Trace metals in human brain tissues and their neuromelanines INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

  29. INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

  30. INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

  31. Biochemical Pharmacology, Aug 1;66(3), pp.489-94, (2003). Pigment Cell Res., 17(6):610-7., Dec; (2004). INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

  32. Fe Cu SN LC LC LC LC LC LC Cu SN INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia Proc Natl Acad Sci U S A.;101(26):9843-8.Epub Jun 21, (2004)

  33. Radiotracers in vivo experiments Nuclear reaction : p, xn Nat Ti 48 V INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

  34. INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

  35. I N R I M I N R I M I N R I M I N R I M Toxic metals in the environment - K and P comparison of CCQM IAWG (2006) INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

  36. I N R I M I N R I M I N R I M I N R I M I N R I M I N R I M As in shellfish INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

  37. FOOD ANALYSIS : TRACE METALS DETERMINATION IN TOMATO PASTE Analytical techniques and procedures for Cadmiun determination INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

  38. INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

  39. INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

  40. Decreto Ministeriale n° 60 del 02/02/2002 with PM10 concentration more than 75 mg/m3 …

  41. Pre-impactor (> 9.0 mm) Stage 1 (6,0 – 9,0 mm) Stage 2 (4,6 – 6,0 mm) Stage 3 (3,3 – 4,6 mm) Stage 4 (2,15 – 3,3 mm) Stage 5 (1,08 – 2,15 mm) Stage 6 (0,70 – 1,08 mm) Stage 7 (0,41 – 0,70 mm) Stage 8 (0 – 0,41 mm)

  42. Air particulate matter collected onto filters Stage 1(6.0-9.0 m) Stage 5(1.08-2.15 m) Total Suspended Particle (TSP) Stage 8 (0-0.41 m)

  43. Reference Materials for life science : Trace elements in air particulate matter PM 2.5 NIST 2783 Air Particulate on Filter Media Lab. 7: IMGC Lab.12: NIST INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

  44. NIST 2783 Air Particulate on Filter Media Lab. 7: INRIM Lab.12: NIST Reference Materials for life science : Trace elements in air particulate matter PM 2.5 INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

  45. Gaithersburg, MD 20899 Certificate Issue Date: 2 January 2002 INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

  46. YES!! INRIM Unit of Radiochemistry and Spectroscopy c/o University of Pavia

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