1 / 31

Effectiveness of Irradiation in Controlling Pathogenic and Spoilage Microorganisms in Meats

Overview. Effect of irradiation on pathogenic and spoilage microorganismsEffect of irradiation on shelf life of fresh meatsTypes of irradiated meat productsFuture research needs. Biological effects of irradiation on microorganismsFactors affecting irradiation effectiveness against microorganismsRadiation resistance.

Rita
Download Presentation

Effectiveness of Irradiation in Controlling Pathogenic and Spoilage Microorganisms in Meats

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

    2. Overview Effect of irradiation on pathogenic and spoilage microorganisms Effect of irradiation on shelf life of fresh meats Types of irradiated meat products Future research needs

    3. Biological effects of irradiation on microorganisms Factors affecting irradiation effectiveness against microorganisms Radiation resistance Effect of irradiation on pathogenic and spoilage microorganisms

    4. Biological effects of irradiation on microorganisms Irradiation can directly impair critical cell functions or components (DNA) Single strand breaks (repairable in most cases) Double strand breaks (not repairable in most cases)

    6. Irradiation can indirectly form radiolytic products/free radicals from water (oH, oOH) oOH radicals are responsible for 90% of DNA damage Biological effects of irradiation on microorganisms

    7. Biological effects of irradiation on microorganisms Factors affecting irradiation effectiveness against microorganisms Radiation resistance Effect of irradiation on pathogenic and spoilage microorganisms

    8. Factors affecting irradiation effectiveness against microorganisms Growth phase: Some microorganisms (L. monocytogenes) are more susceptible to irradiation at low doses when irradiated during the logarithmic phase of growth then during the stationary phase

    9. Type of food The more complex the food, the greater the competition of the food for the energy and less for the microorganisms Fat content of ground beef does not change the dose needed to eliminate E. coli O157:H7 C. jejuni was more resistant to irradiation in low fat frozen beef Factors affecting irradiation effectiveness against microorganisms

    10. Moisture content The lower the water activity in a food, the less free radicals produced by water--> less DNA damage to microorganisms Low water activity will reduce the ability of radiation resistant organisms to recover during storage Factors affecting irradiation effectiveness against microorganisms

    11. Temperature of food Higher temperatures enhance the lethal effect of irradiation Microorganism repair mechanisms are impaired at the higher temperatures Freezing immobilizes and prevents diffusion of free radicals to microorganisms Factors affecting irradiation effectiveness against microorganisms

    12. Presence of oxygen Higher oxygen concentration = greater lethal effect of radiation on microorganisms Anaerobic conditions = less lethal effect of radiation on microorganisms Storage conditions can enhance recovery of some microorganisms after irradiation Factors affecting irradiation effectiveness against microorganisms

    13. Biological effects of irradiation on microorganisms Factors affecting irradiation effectiveness against microorganisms Radiation resistance Effect of irradiation on pathogenic and spoilage microorganisms

    14. Approximate doses of radiation needed to kill various organisms

    15. Radiation resistance Gram negative bacteria < Gram positive bacteria/molds/yeast < spores and viruses Differences in resistance are due to: chemical and physical structure of microorganism ability to recover from radiation injury

    16. Effect of Irradiation on Pathogens Population (log10 CFU/g) - killed by :

    17. Radiation resistance Deinococcus radiodurans is highly resistant to irradiation Acid resistant E. coli O157:H7 exhibit radiation resistance Of pathogens tested in ground beef, C. jejuni has lowest resistance to irradiation; Salmonella spp. has the highest resistance

    18. Typical radiation survival curves

    19. Radurization Irradiation applied to elicit a substantial reduction in number of spoilage organisms, thereby extending the shelf life of a food 3-4 times Applied at dosage of 0.5-10 kGy 5 kGy will eliminate most spoilage organisms

    20. Radicidation Irradiation is applied to reduce the number of non-spore forming pathogenic microorganisms (other than viruses) and parasites Applied at dosage of 3.0-10 kGy Improves the hygienic quality of the food Reduces the risk of public exposure to pathogens

    21. Radappertization Irradiation is applied to prepackaged, enzyme-inactivated foods to reduce the number and/or activity of microorganisms (12-D reduction in C. botulinum spores) Applied at dosage of 25-60 kGy In the absence of post-processing contamination, no microbial spoilage or toxicity should occur Shelf stable without refrigeration

    22. Overview Effect of irradiation on pathogenic and spoilage microorganisms Effect of irradiation on shelf life of fresh meats Types of irradiated meat products Future research needs

    23. Effect of irradiation on shelf life of fresh meats Spoilage organisms, especially pseudomonads, are susceptible to low dose irradiation Spoilage of low dose irradiated meats may be due to yeast, LAB, or Moraxella spp. (increased lag time)

    25. Overview Effect of irradiation on pathogenic and spoilage microorganisms Effect of irradiation on shelf life of fresh meats Types of irradiated meat products Future research needs

    26. Types of meat products approved for irradiation Refrigerated or frozen, uncooked meat or meat byproducts Meat from cattle, sheep, swine, or goats, which is skeletal or which is found in the tongue, diaphragm, heart, or esophagus, with or without the accompanying and overlying fat. (It does not include the muscle found in the lips, snout, or ears.)

    27. Types of meat products approved for irradiation Mechanically deboned meat Intact or ground meat Hamburger Certain defatted beef or pork products

    28. Overview Effect of irradiation on pathogenic and spoilage microorganisms Effect of irradiation on shelf life of fresh meats Types of irradiated meat products Future research needs

    29. Future research needs Determination of processes (heat, antimicrobials, curing agents, etc.) that may affect radiation resistance of pathogens in fresh meats The effect of multi-hurdle approach with irradiation to enhance pathogen reduction or improve shelf life in fresh meats

    30. Future research needs Determining effects of irradiation on different cooked meat products to inhibit organisms such as LM Determining effects of atmospheres and packaging regimens on pathogens associated with cooked and fresh meats subjected to low dose irradiation

    31. Societal Benefits of Irradiating Ground Beef If 25% of ground beef supply (~2 billion pounds) is irradiated: it would cost $88.5 million (assuming $0.05/lb); $28.3 million (assuming $0.02/lb) 25% of Salmonella spp. and E. coli O157:H7 infections would be prevented for saved medical costs of $56 million to $138 million

    32. QUESTIONS?

More Related