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DNA FINGERPRINTING. The story…. 1) Provide a timeline 2) Draw a family tree 3) How could you explain Tom’s resemblance to TJ? 4) Why is the Y chromosome a good marker? 5) What is a haplotype ? 6)How can the descendants be used to indicate paternity?. DNA. Double helix A = T C = G.
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DNA FINGERPRINTING
1) Provide a timeline2) Draw a family tree3) How could you explain Tom’s resemblance to TJ?4) Why is the Y chromosome a good marker?5) What is a haplotype?6)How can the descendants be used to indicate paternity?
DNA • Double helix • A = T • C = G
Restriction Enzymes • Bacterial origin • Cuts DNA sequences into shorter fragments • Site specific • Ex: EcoR1 cuts at GAATTC sequence Living organisms that have different characteristics The more different the organisms the more different are the DNA sequences different DNA sequences
www.uic.edu/classes/phar/phar331/lecture6/ Restriction Enzymes
DNA FINGERPRINTING Professor Sir Alec Jeffreys http://www.biotech.iastate.edu/biotech_info_series/bio6.html#anchor861530#anchor861530
DNA Fingerprinting- Who’s the father? Are you sure?
Use Multiple Probes Eric S. Lander