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Organizing the cell: the Cytoskeleton. Intermediate Filaments. Microfilaments. Microtubules. Microtubules. Text and image sources are included using the notes function of this file. EM views. FtsZ: a prokaryotic ur-tubulin?. The Tubulin Superfamily. Microtubule model. exchangeable. GDP.
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Organizing the cell: the Cytoskeleton Intermediate Filaments Microfilaments Microtubules
Microtubules Text and image sources are included using the notes function of this file
Microtubule model exchangeable GDP GTP “Minus” end “Plus” end GTP GTP GTP GTP GDP GTP GTP GTP GDP GDP GDP GDP GDP GDP GTP GTP GTP GTP GTP GTP GTP GTP GDP GTP GTP Beta-tubulin “cap”
Plus Minus Tubulin dimer atomic structure In Out
Microtubule fly-by Narrated Video
The Centrosomerevealed 9-triplet microtubule centrioles pericentriolar material singlet microtubules
Washout of nocodazole 10 um Successive images at 2 minute intervals after changing to drug-free media (at timepoint A)
Disassembly Stathmin microtubule-binding domain Video In vitro assembly on axonemal microtubule seeds +/- Stathmin Activity
Katanin Video Microtubule wall (Mitotic Extract) (Interphase Extract)
Treadmilling Speckles Photobleaching
MAPs Note the cross-bridges (arrows) between microtubules
Plus end tracking proteins (+TIPS) Video Video GFP::EB1 Cy3 Tubulin um
Interrelationship between microtubules and microfilaments Microtubule effects Microfilament effects Video Video
Synthesis and Folding Signal transduction Integration with actin networks cof B cof E cof C CCT cof D cof A Pathways stathmin PAK Rac Cdc42 a b Rho IQGAP abGTP • Tub; CLIP-170, EB-1 mDia GEF? Dynamic Polymer Rho stabilizing MAPs; + end capture stathmin catastrophic kinesins abGDP katanin severin (EF1a) StablePolymer Assembly/Disassembly