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SEED HEALTH COMMITTEE

Sunday, 20 June 2010. Theresa Aveling University of Pretoria South Africa. SEED HEALTH COMMITTEE. Theresa Aveling , South Africa. Sunday, 20 June 2010 . 2007-2010 Seed Health Committee. Sunday, 20 June 2010. PAST, PRESENT and FUTURE Rules development Introduction of new methods

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SEED HEALTH COMMITTEE

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  1. Sunday, 20 June 2010 • Theresa Aveling • University of Pretoria South Africa SEED HEALTH COMMITTEE Theresa Aveling, South Africa

  2. Sunday, 20 June 2010 2007-2010 Seed Health Committee

  3. Sunday, 20 June 2010 PAST, PRESENT and FUTURE Rules development Introduction of new methods Introduction of Rules changes Publications Seed health handbook Annotated list of seed-borne diseases Workshops and symposia Proficiency testing Special projects

  4. Sunday, 20 June 2010 PAST and PRESENT: Rules development Introduction of new methods 2007: 7-021 Xanthomonas axonopodis pv. phaseoli and pv. phaseolis var. fuscans on Phaseolus vulgaris 2008: 7-022 Agar method for the detection of Michrodochium nivale on Tritichum spp.

  5. Sunday, 20 June 2010 PAST and PRESENT: Rules development Introduction of new methods 2008: 7-023 Detection of Pseudomonas savastanoi pv. phaseolicola on Phaseolus vulgaris 2008: 7-024 Detection of Pea Early Browning Virus and Pea Seed-borne Mosaic Virus on Pisum sativum

  6. Sunday, 20 June 2010 PAST and PRESENT: Rules development Introduction of new methods 2009: 7-025 Detection of Aphelenchoides besseyi on Oryza sativa 2010: 7-026 Detection of Squash Mosaic Virus, Cucumber Green Mottle Mosaic Virus and Melon Necrotic Spot Virus in cucurbits

  7. Sunday, 20 June 2010 Future 2010-2013: Rules development Introduction of new methods Xanthomonas hortorum pv. carotae on Daucus carota – PCR method Xanthomonas campestris pv. campestris – disinfected Brassica seeds Fusarium spp. on Zea mays Tobamo viruses/pepper and Lycopersicon esculentum – bioassay Pyrenophora spp. on Hordeum vulgare

  8. Sunday, 20 June 2010 Future 2010-2013: Rules development Introduction of new methods Ustilago nuda on Hordeum vulgare (Nordic) Amalgamation of 3 methods for Linum At SHC, it was decided to align the three methods 7-007, 7-017, 7-018 in a single method to detect Alternaria linicola, Botrytis cinerea, and Colletotrichum lini Peer validation included 3  labs: Volcani (R Kohen), SNES (V Grimault) and Canadian Food Inspection Agency (S Brière) The parameters which were different in the three ISTA methods were compared: incubation T°, light during incubation and length of incubation were selected as parameters

  9. A pretest was carried out in one lab at SNES, to define the conditions to be tested for the peer validation. As new ISTA methods include the addition of 50 to 130 mg/L of Streptomycin, we decided to add this parameter for comparison: With/withoutantibiotics: Streptomycin 0; 50; 130 and 200 mg/L at 20°C dark Dark, Light/dark, dark/NUV Temperature: 20, 22°C Lots: 15% per pathogen, 4*100 seeds Results of pretest did not show significant difference between conditions

  10. Sunday, 10 June 2010 Test plan: Peer validation 3 levels of contamination per pathogen (healthy, low, medium) 4*100 seeds per condition 5 Conditions : 1: Malt agar without streptomycin, 20°C, in darkness as 7-007 2: Malt-agar with streptomycin 50 mg/L, 20°C, in darkness during 9 days and 12h NUV/12h dark. 3: Malt agar without streptomycin, 22°C, 12h NUV/12h dark as 7-0017 and 7-0018 4: PDA with streptomycin 50 mg/L, 20°C, in darkness during 9 days and NUV 5: PDA with streptomycin 50 mg/L, 20°C, 12h light, 12h dark Evaluation 5, 7, 9 days

  11. Sunday, 20 June 2010 Results of peer validation: Statisticalanalysis All conditions testedallowdetection and identification of the 3 pathogens We will propose a method based on these conditions for the update of the ISTA methods for detection of A. linicola, C. liniand B. cinerea: 50 ppm Streptomycin 20°C Malt Agar or PDA Dark 9 days and NUV 12h if needed for sporulation Evaluation 5, 7, 9 days

  12. Sunday, 20 June 2010 Future 2010-2013: Rules development Introduction of Rules changes Editorial and update of taxonomical names Consistancy

  13. Sunday, 20 June 2010 PAST, PRESENT and FUTURE: Publications Seed health handbook CHP 1 Introduction CHP 2 Sampling CHP 3 Design & interpretation of seed health tests CHP 4 Basic techniques CHP 5 Testing for fungi CHP 6 Testing for bacteria CHP 7 Testing for viruses CHP 8 Testing for nematodes

  14. Sunday, 20 June 2010 PAST, PRESENT and FUTURE: Publications Seed health handbook CHP 9 Immunology CHP 10 Molecular techniques CHP 11 Quality assurance CHP 12 Seed treatment Glossary Useful references Appendices???

  15. Sunday, 20 June 2010 PAST, PRESENT and FUTURE: Publications Annotated list of seed-borne diseases Large long-term project To start in 2010/2011 Concentrate on crop

  16. Sunday, 20 June 2010 PAST and PRESENT: Workshops and symposia 7-11 April 2008: Seed Health workshop in Pretoria, South Africa.21 participants from 11 countries: Brazil (1), Israel (1), Kenya (1), Nigeria (1), Seychelles (1), South Africa (10), Spain (1), Sudan (1), Tanzania (2), United States (1), Zambia (1)

  17. Sunday, 20 June 2010 PAST and PRESENT: Workshops and symposia (cont.) Instructors: R Blanco (University of Almeria, Spain), K Tylkowska (Agricultural University of Poznan, Poland), C Nieves Mortensen (University of Copenhagen, Denmark), T Goszczynska (Plant Protection Research Institute, South Africa), R Mumford (Central Science Laboratory, United Kingdom)and M Lesufi (Department of Agriculture, South Africa)

  18. Sunday, 20 June 2010 PAST and PRESENT: Workshops and symposia (cont.) ISTA 6th SEED HEALTH SYMPOSIUMBerg en DalRestcamp, Kruger National Park, South Africa, 14-18 April, 2008

  19. Sunday, 20 June 2010 PAST and PRESENT: Workshops and symposia (cont.) Organiser: T Aveling. 1st time in Africa. 65 participants from 28 countries. 22 paper and 14 poster presentations and 2 workshops

  20. Sunday, 20 June 2010 Organisers: V Grimault and V Cockerell 21 participants from 16 countries (Europe, Kenya, Israel, Brazil) PAST and PRESENT: Workshops and symposia (cont.) Detection of Botrytis cinerea on Linum seeds and on sunflower seeds; detection of Fusarium spp. on wheat seeds GEVES-SNES Angers France. 2-5th March 2010

  21. Sunday, 20 June 2010 2 sessions, one on Fusarium and one on Botrytis 11 lectures dealing with epidemiology, taxonomy, detection methods Lectures by scientists from INRA, University of Angers and by specialists from Canada, Scotland and France.

  22. Sunday, 20 June 2010 FUTURE: Workshops and symposia 2011 Seed Health workshop Aleppo, Syria 7th Seed Health Symposium Aleppo, Syria 2012 Possible Workshop in Spain

  23. Sunday, 20 June 2010 PAST and PRESENT: Proficiency testing 7-003 Botrytis cinereaon Helianthus annus 7-013 Ustilagonudaon Hordeumvulgare 7-001a,b and 7-002a,b Alternariadauciand A. radicinaon Daucuscarota 7-020 Xanthomonashortorumpv. carotae on Daucuscarota 7-010, 7-011, 7-012 Dreschleraoryzae, Pyriculariaoryzaeand Alternariapadwickion Oryzae sativa 7-022 Microdochiumspp. on Triticum

  24. Sunday, 20 June 2010 FUTURE: Proficiency testing 7-009 Fusariumcircinatumon Pinustaedaand Pinuselliottii 7-006 Colletotrichumlindemuthianum on Phaseolusvulgaris 7-019 Xanthomonascampestrispv. campestris on Brassica spp. 7-013 Ustilagonudaon Hordeum Depending on availability of contaminated seed ???

  25. Sunday, 20 June 2010 PAST, PRESENT and FUTURE: Special projects Seed health testing sample size: Questionnaire Phytosanitary aspects: Questionnaire Seed treatment

  26. Sunday, 10 June 2010 Collaboration Comparative tests for seed-borne fungal pathogens of spinach in co-operation with Lindsey du Toit of Washington State University Standardization of Clavibactermichiganensis subsp. michiganensis test on tomato seeds in co-operation with EPPO

  27. Sunday, 20 June 2010 2010-2013 Seed Health Committee

  28. THANK YOU!

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