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Good afternoon, Agents.

Good afternoon, Agents. Please take out a writing utensil and place your backpack below your desk. Crime Scene Investigation: Macromolecule Identification . Period 4 Debriefing Meeting. Crime scene #UNI091811.

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Good afternoon, Agents.

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  1. Good afternoon, Agents. Please take out a writing utensil and place your backpack below your desk.

  2. Crime Scene Investigation: Macromolecule Identification Period 4 Debriefing Meeting

  3. Crime scene #UNI091811 At 3:15 PM on Friday, September 16th 2011, an iPod belonging to Jane Doe was stolen. Remnants of food foundat the scene have been taken as evidence. Four suspects have been questioned, and food items from their lockers confiscated.

  4. LIBRARY 9/18/2011 ♬ ♪ ♫ CASE #UNI091811

  5. CASE #UNI091811

  6. . . . . . . . . . . CASE #UNI091811

  7. . . . . . CASE #UNI091811

  8. !!?! CASE #UNI091811

  9. Sketch by crime artist

  10. Your task is to identify the food item in the evidence based on your biochemistry knowledge of macromolecules.

  11. So what do you know about macromolecules? monosaccharide fatty acid polysaccharide CARBOHYDRATES LIPID nucleotide amino acid NUCLEIC ACID PROTEIN

  12. Each team will be given 3 types of samples: • Dry evidence • Liquid evidence • One food sample from the suspects:

  13. You have been assembled into color-coded teams.

  14. The results of each team’s suspect food data will be compiled at 1:15 PM with our second debriefing meeting. From this meeting, you will be able to identify your unknown evidence and single out the culprit.

  15. We will take the time now to assign a number 1-6 to each agent in your team. Agent #1: Polysaccharide expert Agent #2: Monosaccharide expert Agent #3: Protein expert Agent #4: Lipid expert Agent #5: Recorder Agent #6: Recorder

  16. Agent #1: Polysaccharide expert You will use Iodide to determine the presence of starch.

  17. BASIC PROCEDURE • Put on lab apron, gloves, and goggles. • Label test tubes • Pour out dry evidence into weigh boat. Use back end of spoon to scoop and pour evidence. • Add water to the line, tap to mix well. • Add biochemical indicator, tap to mix well. • Relay observation to recorder.

  18. Agent #1: Polysaccharide expert Add 5 drops of Iodide to determine the presence of starch. Iodide solution changes from clear orange to deep blue-black in presence of starch

  19. Agent #2: Monosaccharide expert Add 8 drops of Benedict’s solution to determine the presence of glucose. Benedict’s solution changes from clear blueto thick yellow-orange-redin presence of glucose

  20. Agent #3: Protein expert Add 8 drops of Biuret solution to determine the presence of protein. Biuret’s solution changes from clear light blueto lavender-brownin presence of protein

  21. Agent #4: Lipid Expert Smear the liquid sample onto a paper bag to determine the presence of lipids. Only you will be responsible for the LIQUID evidence.

  22. You will have until 1:27 PM to complete this investigation. Good luck teams.

  23. Return unused dry evidence back into tube • Place dirty test tubes into water tub—don’t worry about gunk! • Throw away lipid brown papers • Return racks, water bottles into shoe box. CASE #UNI091811

  24. Actual Samples and Evidence Polysaccharide test Monosaccharide test Protein test

  25. Thank you scientists!

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