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Drosophila Genetics

Drosophila Genetics. Inheritance of Body Color B. Three Point Testcross. Fruit Fly Egg and Larva. Fruit Fly Pupa and Adult. Inheritance of Body Color: Phenotypes of Parents. Inheritance of Body Color: Crosses. A x C B x C D x C A x E B x E D x E

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Drosophila Genetics

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  1. DrosophilaGenetics • Inheritance of Body Color • B. Three Point Testcross

  2. Fruit Fly Egg and Larva

  3. Fruit Fly Pupa and Adult

  4. Inheritance of Body Color: Phenotypes of Parents

  5. Inheritance of Body Color: Crosses A x C B x C D x C A x E B x E D x E Score each fly for gender and body color Yellow Beige

  6. Distinguishing Male and Female Fruit Flies • Male body size is smaller than female • Male abdomen is tapered and darker than female abdomen • Males have sex combs at first joint on front legs You MUST be able to see sex combs on any fly you identify as male!

  7. bl+ vg+ bw+ bl vgbw X PARENTALS: bl+ vg+ bw+ bl vgbw bl+ vg+ bw+ F1: bl vg bw Three Point Testcross

  8. Black, vestigial, Brown Males F1 Females bl+ vg+ bw+ bl vg bw X bl vg bw bl vg bw F2 Generation Three Point Testcross

  9. Three Point Testcross: Possible Phenotypes

  10. Three Point Testcross: F2 Offspring Score each fly for body color, wing shape and eye color.

  11. Wild Type Versus Mutants • Body Color • Wild type: Beige • Mutant: Black

  12. Wild Type Versus Mutants • Wing Shape • Wild Type: Long • Vestigial

  13. Wild Type versus Mutant • Eye Color • Wild Type: brick red • Mutant: Brown “red-brown”(slightly lighter than sepia)

  14. Nap Time

  15. How to anesthetize a fly

  16. Viewing the Fruit Fly

  17. Reference for Pictures: • Carolina Drosophila Manual (45-2620) • Published by Carolina Biological Supply Company

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