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Drosophila melanogaster. Dorsal. Posterior. Anterior. Ventral. Cleavage in the Drosophila Embryo. Embryo. A. P. Larva. A. P. P. A. 2 hrs. A. P. 10 hrs. A. P. A. P. Genetic Hierarchy in A-P Axis Specification. Immunostaining. Dye-conjugated antibody. Protein.
E N D
Dorsal Posterior Anterior Ventral
Embryo A P Larva A P
P A 2 hrs. A P 10 hrs.
A P A P
Genetic Hierarchy in A-P Axis Specification
Immunostaining Dye-conjugated antibody Protein
Bicoid is Sufficient for Anterior Structure Formation
Bicoid Hunchback (hb) Promoter
Gap Gene Expression Patterns
Pair-rule Gene Expression Pattern in Syncytial/Cellular Blstoderm 14 13 12 1 11 2 3 10 4 9 5 8 6 7 Parasegments Eve = Blue Ftz = Brown
Eve Stripe 2 (parasegment 3) Regulatory Region
Segment Polarity Genes Engrailed (en) Gene Expression
Wild Type wg mutant segment
Synpolydactyl Phenotype HoxD13 mutation
Extra-embryonic tissue Dorsal ectoderm (epidermis) Ventral ectoderm (nervous system) Mesoderm
Neural Tube Dorsal Ventral
Neuron Specification Shh = Sonic hedgegog (signal molecule)