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H 14 CO 3 -

0. 0.25. 0.5. 0.75. 1. CPS. ATCase. 14 Carbamoyl Aspartate. 14 Carbamoyl Phosphate. H 14 CO 3 -. Gln, H 2 O, ATP. Asp. “cold” Carbamoyl Aspartate. “Cold” Carbamoyl Phosphate. DA Linker. DHO. ATCase. CP S ase. Wild-Type. C. N. CP S ase. ATCase.

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H 14 CO 3 -

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  1. 0 0.25 0.5 0.75 1 CPS ATCase 14Carbamoyl Aspartate 14Carbamoyl Phosphate H14CO3- Gln, H2O, ATP Asp “cold” Carbamoyl Aspartate “Cold” Carbamoyl Phosphate DA Linker DHO ATCase CP Sase Wild-Type C N CP Sase ATCase DHO Without DA Linker (DAD) N C Wild-type DAD 5 5 4 4 14Carbamoyl Aspartate (mM) 3 3 Observed ( ) Theoretical ( ) 2 2 Observed ( ) 1 1 Theoretical ( ) 0 0.25 0.5 0.75 1 Exogenous (“cold” )Carbamoyl Phosphate (mM) GLU GLU DHO DHO DA Linker GLN GLN NH3 NH3 CAsp CAsp HCO3- HCO3- CAsp CBX CBM CBX CBM CAsp CP CP ATP ATP CP CP CP ASP ATP ATP ASP Channeling of CP(Wild-type) No Channeling of CP(DAD) Adapted from Guy&Evans, J.Biol.Chem (1994), V269:23808-16

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