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Linkage Mapping

Linkage Mapping. P. 280. Linkage Mapping. Problem 1: Mating: AaBb x aabb A = Long antennae a = Short antennae B = Green eyebrows, and b = Blue eyebrows.  How do you tell your genes are linked? Prediction for 2000 offspring?. Problem 1 Results. Long Green - 850 Long Blue - 150

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Linkage Mapping

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  1. Linkage Mapping

  2. P. 280

  3. Linkage Mapping • Problem 1: Mating: AaBb x aabb • A = Long antennae • a = Short antennae • B = Green eyebrows, and • b = Blue eyebrows. •  How do you tell your genes are linked? • Prediction for 2000 offspring?

  4. Problem 1 Results • Long Green - 850 • Long Blue - 150 • Short Green - 150 • Short Blue - 850 • (Total # crossovers/total # offspring) x 100 = LMU (or cM).

  5. Problem 2 – Autosomal Mapping • Drosophila: • Wing shape – wild/arc • Body color – wild/black • Progeny: • 660 wild • 530 black • 490 arc • 712 arc black

  6. Problem 3: Sex-linked cross • Sex linked: • eye color – wild type/white • Body color – wild type/yellow • Male Progeny: • Yellow white – 43 • White – 2146 • Yellow – 2302 • Wild - 22

  7. Problems for additional practice: • Example: p. 280 • p. 291, #4 • p. 291, #5 • Online textbook quiz questions for Ch. 15

  8. Problem 4: Three point test cross • Corn: • Growth habit – normal/lazy • Leaf appearance – normal/glossy • Endosperm – normal/sugary • Progeny: • 286 wild • 272 lazy glossy sugary • 40 glossy sugary • 33 lazy • 44 lazy sugary • 59 glossy • 2 lazy glossy • 4 sugary

  9. Problem 5 - Imaginary Critters • D = Calm personality • d = Dithery personality • F = Five toed • f = Four toed • G = Smooth fur, and • g = Grizzled fur. Progeny: Calm, Five, Smooth – 616 Dithery, Five, Smooth - 5 Calm, Five, Grizzled – 104 Dithery, Five, Grizzled - 75 Calm, Four, Smooth – 78 Dithery, Four, Smooth - 100 Calm, Four, Grizzled – 2 Dithery, Four, Grizzled - 620

  10. Problem 6 - Groodies • Groodies are useful (but fictional) haploid organisms that are pure genetic tools. A wild-type groody has a fat body, a long tail, and flagella. Mutant lines are known that have thin bodies, or are tailless, or do not have flagella. Groodies can mate with each other (although they are so shy that we do not know how) and produce recombinants. A wild-type groody mates with a thin-bodied groody lacking both tail and flagella. The 1000 baby groodies produced are classified as shown in the following illustration. Assign genotypes, and map the three genes. (Problem 18 from Burton S. Guttman.)

  11. Assign genotypes, and map the three genes.

  12. Problems for additional practice: • p. 292, #10 & 11 • Online textbook quiz questions for Ch. 15

  13. Constructing a Linkage Map

  14. Problem 7 – Chromosomal Linkage Mapping • Four genes on chromosome: ABCD • Linkage data: • A & B = 7 LMU • A & C = 6 LMU • A & D = 4 LMU • B & C = 1 LMU • B & D = 11 LMU • C & D = 10 LMU

  15. Problem 8 – Chromosomal Linkage Mapping • Four genes on chromosome: ABCD • Linkage data: • A & B = 17 LMU • A & C = 7 LMU • A & D = 13 LMU • B & C = 10 LMU • B & D = 4 LMU • C & D = 6 LMU

  16. Problem 9 – Chromosomal Linkage Mapping • Five genes on chromosome: ABCDE • Linkage data: • A & B = 3 LMU • A & C = 11 LMU • A & D = 9 LMU • A & E = 9 LMU • B & C = 8 LMU • B & D = 6 LMU • B & E = 12 LMU • C & D = 2 LMU • C & E = 20 LMU • D & E = 18 LMU

  17. Problems for additional practice: • p. 292, #8 • Online textbook quiz questions for Ch. 15 • http://www.tinyurl.com/linkagemap

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