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Safe Working Practices Guidelines for Honours Students: General Safety Precautions

Stay safe with these specific rules for lab practices: general safety precautions, glassware handling, chemical preparation, hazardous waste disposal, autoclaving procedures, WHMIS guidelines, and responsibilities.

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Safe Working Practices Guidelines for Honours Students: General Safety Precautions

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  1. Safe Working Practices for Honours, Directed Studies Students

  2. General Safety Precautions • General Rules • Lab Coat • Footwear • Handwashing • No Eating in Lab Area • Personal Belongings • Fire Procedure • Injuries • Emergency Number

  3. Biohazardous Materials • Do not remove plates etc from lab • Waste plates • Waste non disposals • Spills • Nothing Goes Down The SINK Unprocessed • Disinfect work area • Spill cleanup • Wash hands before leaving

  4. Chemical Preparation • Check MSDS for proper handling • Labeling and storage • PPE • Order needed • Safe Disposal

  5. Disposal • Glassware • Broken glass • Autoclaving • Hazardous wastes

  6. Glassware • Rinse and ensure glassware is immersed in water in bucket in washup area • When necessary do a load in dishwasher • Return to appropriate shelf etc

  7. Broken Glass • Not contaminated with biohazard in glass breakage containers • Biohazard contaminated: yellow sharps containers • Leave note on white board in S365C if broken glassware

  8. Autoclaving • Waste biohazards • Liquids • Use slow exhaust • Wrapped • Allow for drying cycle • Autoclave tape • Check with others before starting (particularly Carolynne)

  9. Hazardous Waste • Container • Appropriate size • Labeling • Contains all ingredients (no abbreviations) • Location • S365F

  10. Before Leaving After Every Session • Clean any areas that have been used (balances, counter, fumehood) • Glassware is put in washup area • Turn off gas • Turn off deionized water • Turn off lights • Turn off autoclave • Lock door

  11. Working Alone • If at all possible avoid it • A risk assessment of your work must be done with your supervisor to determine what can be done with minimum supervision • Higher risk procedures should not be done when alone (working with hazardous materials, risk of fire) • http://www.ccohs.ca/oshanswers/hsprograms/workingalone.html

  12. Working Alone • The Faculty of Science will be striking a multidiscipline committee to develop working alone guidelines for the Faculty of Science • Have some type of checkin procedure

  13. Safe Work Procedures • http://www.tru.ca/hsafety/workinglearningsafely/work.html

  14. WHMIS

  15. What is WHMIS • Workplace Hazardous Materials Information System • Provides information about hazardous materials used in the workplace • Workers have the right to receive information about each controlled product they use • Goal of WHMIS is to reduce injury and disease.

  16. HAZARD CLASSES • Class A: Compressed Gas • Class B: Flammable & Combustible • Class C: Oxidizing Material • Class D: Poisonous & Infectious Material

  17. HAZARD CLASSES • Class E: Corrosive Material • Class F: Dangerously Reactive Material

  18. SUPPLIER LABELS Seven items of information must be included on supplier label: • Product identifier • Hazard Symbols • Risk Phrase • Precautionary Statements • First Aid Measures • Supplier Identification • Reference to MSDS

  19. WORKPLACE LABELS Required on containers for each controlled product on site, including secondary containers. Provide 3 types of information: • Product Identifier • Safe Handling and personal protective clothing and equipment. • Reference to MSDS

  20. WORKPLACELABEL

  21. MSDS SHEET • Material Safety Data Sheet • provides specific hazard information, safe handling information, and emergency procedures for a controlled product. • Key source of information for developing safe working procedures.

  22. MSDS SHEETS Nine Recommended Section Headings: • Product Information • Hazardous Ingredients • Physical Data • Fire and Explosion Hazard • Reactivity Data • Toxicological Properties • Preventative Measures • First Aid Measures • Preparation Information

  23. Cycloheximide MSDS • Routes of Entry: Absorbed through skin. Eye contact. Inhalation. Ingestion. • Toxicity to Animals: Acute oral toxicity (LD50): 2 mg/kg [Rat]. • Chronic Effects on Humans: • DEVELOPMENTAL TOXICITY: Classified Reproductive system/toxin/female, Development toxin [POSSIBLE]. • Causes damage to the following organs: the nervous system, liver.

  24. Cycloheximide • May cause damage to the following organs: blood, kidneys, gastrointestinal tract, skin. • Other Toxic Effects on Humans: Very hazardous in case of skin contact (irritant), of ingestion, of inhalation.

  25. TRU MSDS • Bookmark student computer S365B • ThompsonMSDS • Msds • Read before using new chemicals

  26. RESPONSIBLITIES Workers • Know & understand info on labels and MSDS • Handle controlled products safely. • Inform employer if labels missing Employer • Ensure workers understand WHMIS • Provide training • Ensure controlled products have MSDS and WHMIS Labels • MSDS available to workers

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