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Biotechnology/Cloning. Reproductive cloning. Reproductive cloning. Therapeutic cloning. Therapeutic cloning. http://www.rndsystems.com/dam_public/6515.jpg. Recombinant DNA technology. DNA isolated from two sources and cut by the same enzyme. Plasmid (vector). Gene of interest.
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Biotechnology/Cloning Reproductive cloning
Therapeutic cloning http://www.rndsystems.com/dam_public/6515.jpg
Recombinant DNA technology DNA isolated from two sources and cut by the same enzyme Plasmid (vector) Gene of interest Some uses of genes: Some uses of proteins: Recombinant DNA Gene for pest resistance Protein for dissolving clots Gene for toxic-cleanup bacteria Protein for “stone-washing” jeans Transgenic organisms Useful products
First Cloning Experiment Cohen et al., 1973 “Recombinant DNA”
Cloning into pBR322 Cloning Vectors: pBR322 plasmid
Palmiter et al. (1982) Dramatic growth of mice that develop from eggs microinjected with metallothionine-growth hormone fusion genes. Nature 300: 611-615 “Supermice”
First Glowing Pet Weeding Out Land Mines