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T Cell Isolation

In patient's T Cell Isolation, flow cytometry analysis of isolated blood products ensures that a sufficient number of healthy and functional T cells being used for processing and re-infusion. https://www.creative-biolabs.com/car-t/flow-cytometry-application-in-car-t-system.htm

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T Cell Isolation

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  1. Flow Cytometry Application in CAR-T System Reference: https://www.creative-biolabs.com/car-t/flow-cytometry-application-in-car-t-system.htm

  2. Background • Flow cytometry is a widely used laser- or impedance-based method for analyzing the expression of cell surface and intracellular molecules, characterizing and defining different cell types in a heterogeneous cell population, assessing the purity of isolated subpopulations and analyzing cell size and volume. It allows simultaneous multi-parameter analysis of the physical and chemical characteristics of up to thousands of particles per second at single cell level.

  3. Flow Cytometry Application • ➢ Patient's T Cell Isolation Flow cytometry analysis of isolated blood products ensures that a sufficient number of healthy and functional T cells being used for processing and re-infusion. • ➢ CAR Expression Evaluation Assure that a sufficient number of viable T cells are expressing the specific CAR, and make sure that these cells show other phenotypic indicators of T cell functionality. • ➢ CAR-T Cell Activation and Expansion Track T cell activation and expansion with cell proliferation and viability dyes in an easy way.

  4. Flow Cytometry Application • ➢ CAR-T Cell Characterization analyze the phenotypic and subset composition of CAR-T cell populations. • ➢ CAR-T Cell Function Monitoring monitor the persistence and functionality of these CAR-T cells as the evaluation of effectiveness of this treatment. It can be applied in functional cytokine production, cytokine release assay, immunohistochemistry analysis of CAR-T cell infiltration in tumor microenvironment, common immuno-activated or immunosuppressive markers expression etc.

  5. Flow Cytometry Application • ➢ T Cell Exhaustion examine T cell exhaustion with markers including PD-L1 and FoxP3. Low exhausted phenotype ensures a more consistent and efficacious product. • ➢ Tumor Regression Monitoring detect the frequency of target cell lysis/apoptotic cells, antitumor activity of CAR-T cell, clinical phenotypes of patients, disease diagnosis etc.

  6. Referenc

  7. Thanks Reference: https://www.creative-biolabs.com/car-t/flow-cytometry-application-in-car-t-system.htm

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