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Use of the Labnet Micropipettor

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Use of the Labnet Micropipettor

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    2. Purpose of the Pipettor Biotechnologists use a pipettor to transfer precise amounts of liquids from one container to another. The measurements are in microliters 1 ml=1000 l.

    3. Care of the Pipettor Misuse of the pipettor can result in incorrect measurements affecting your investigation. Do not drop the pipettor. Do not adjust the volume to less than 2 l.

    4. Parts of the Pipettor 1) yellow pushbutton 2) calibration knob 3) digital volume display 4) barrel 5) disposable pipette tip 6) tip ejector

    5. Volume Adjustment Step 1 In the display window are three vertical numbers. The top number is tens l (10.0). The middle number is ones l (02.0). The bottom number tenths l (00.9).

    6. Volume Adjustment Step 2 Set the pipettor to the desired volume by turning the calibration knob until the volume display reads the desired numbers. 7.5 l would read from top to bottom in the display window: 0 - tens 7 - ones .5 - tenths

    7. Transferring Liquids Step 1 Adjust the pipettor to the desired volume. Hold the pipettor so the display is facing the user and the thumb can easily depress the yellow pushbutton.

    8. Transferring Liquids Step 2 Open the box of yellow pipette tips supplied by the Office of Biotechnology. Place the pipette cone (end) into one of the tips and press down firmly. NEVER USE THE PIPETTOR WITHOUT A TIP!

    9. Transferring Liquids Step 3 Lift the pipettor and attached tip away from the tip box. Depress the pushbutton to the first stop before placing the pipette tip in the liquid. Place the tip in the liquid.

    10. Transferring Liquids Step 4 Slowly release the pushbutton and draw the liquid into the tip. Remove the tip from the liquid and visually check for liquid in the tip.

    11. Transferring Liquids Step 5 Place the tip into the container which is to receive the liquid. Slowly depress the pushbutton as far as it can go. With the pushbutton fully depressed, withdraw the tip.

    12. Transferring Liquids Step 6 Press down on the tip ejector button to eject the tip. Dispose of the tip in an appropriate container.

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