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Use of the Labnet Micropipettor

Use of the Labnet Micropipettor. Purpose of the Pipettor. Biotechnologists use a pipettor to transfer precise amounts of liquids from one container to another. The measurements are in microliters 1 ml=1000 µl. Use of the Labnet Micropipettor. Care of the Pipettor.

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Use of the Labnet Micropipettor

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  1. Use of the Labnet Micropipettor

  2. Purpose of the Pipettor • Biotechnologists use a pipettor to transfer precise amounts of liquids from one container to another. • The measurements are in microliters 1 ml=1000 µl. Use of the Labnet Micropipettor

  3. Care of the Pipettor • Misuse of the pipettor can result in incorrect measurements affecting your investigation. • Do not drop the pipettor. • Do not adjust the volume to less than 2 µl. Use of the Labnet Micropipettor

  4. Parts of the Pipettor 1 1 2 • 1) yellow pushbutton • 2) calibration knob • 3) digital volume display • 4) barrel • 5) disposable pipette tip • 6) tip ejector 6 3 6 6 3 4 4 5 5 Use of the Labnet Micropipettor

  5. Volume AdjustmentStep 1 • In the display window are three vertical numbers. • The top number is tens µl (10.0). • The middle number is ones µl (02.0). • The bottom number tenths µl (00.9). Use of the Labnet Micropipettor

  6. Volume AdjustmentStep 2 • Set the pipettor to the desired volume by turning the calibration knob until the volume display reads the desired numbers. • 7.5 µl would read from top to bottom in the display window: 0 - tens 7 - ones .5 - tenths Use of the Labnet Micropipettor

  7. Transferring LiquidsStep 1 • Adjust the pipettor to the desired volume. • Hold the pipettor so the display is facing the user and the thumb can easily depress the yellow pushbutton. Use of the Labnet Micropipettor

  8. Transferring LiquidsStep 2 • Open the box of yellow pipette tips supplied by the Office of Biotechnology. • Place the pipette cone (end) into one of the tips and press down firmly. • NEVER USE THE PIPETTOR WITHOUT A TIP! Use of the Labnet Micropipettor

  9. Transferring LiquidsStep 3 • Lift the pipettor and attached tip away from the tip box. • Depress the pushbutton to the first stop before placing the pipette tip in the liquid. • Place the tip in the liquid. Use of the Labnet Micropipettor

  10. Transferring LiquidsStep 4 • Slowly release the pushbutton and draw the liquid into the tip. • Remove the tip from the liquid and visually check for liquid in the tip. Use of the Labnet Micropipettor

  11. Transferring LiquidsStep 5 • Place the tip into the container which is to receive the liquid. • Slowly depress the pushbutton as far as it can go. • With the pushbutton fully depressed, withdraw the tip. Use of the Labnet Micropipettor

  12. Transferring LiquidsStep 6 • Press down on the tip ejector button to eject the tip. • Dispose of the tip in an appropriate container. Use of the Labnet Micropipettor

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