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Magnetic Tweezer System Development

Develop a microrheometer to quantify cell-cell adhesion mechanics. Measure viscoelastic parameters of adhesion protein linker systems. Use a magnetic tweezer-based device for perturbing and analyzing mechanics. Accomplished software development, prototype fabrication, and strategy validation. Current phase focuses on size, mass, and complexity reduction. Project timeline includes component review, relationship determination, method selection, and prototype refining.

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Magnetic Tweezer System Development

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  1. Magnetic Tweezer System Development Probing mechanical properties across multiple scales Jason Sherfey Senior BME, Vanderbilt University Advisor: Dr. Franz Baudenbacher

  2. Project Definition • Goal: to design, fabricate, test and miniaturize a microrheometer to quantify cell-cell adhesion • Topics to Cover: Strategy, Reduction & Integration, Current Status, Project Schedule

  3. Strategy • Property measured: The cell-cell adhesion mechanics targeted consist of viscoelastic parameters of adhesion protein linker systems (static & dynamic responses) • Strategy: A magnetic tweezer – based device to perturb, image, and analyze linker system mechanics.

  4. Force 1 nN 3 2 T=0 s displacement [mm] 1 0 0 1 2 3 Time [s] F T=1.5 s Force displacement measurements on magnetic beads linked to the cell surface through E-Cadherin Fit to Mechanical Analog Extract Model parameter

  5. Accomplishments • Developed 1st generation MPT software • Fabricated magnetic tweezer and constructed 1st prototype • Established protocol to implement prototype • Validated strategy by experiment • Refined PT, MT, & protocol parameters to increase SNR & temporal resolution

  6. Current Status • On schedule • Effectiveness of strategy is confirmed • System parameters critical to the accuracy and reproducibility of acquired information have been identified and refined • The final phase of development involves reducing system size, mass, and complexity constrained by the desired critical system parameters

  7. Project Schedule • 1/29, Finish list of components that can be reduced, eliminated or subsume • 2/1, Determine relationship b/w listed mods and system parameters • 2/5, Finish list of methods to implement “allowable” mods (brainstorm, lit review, ..) • 2/8, Selected 2-3 best methods (minimize complexity & cost; max practicality) • 2/15, Have chosen 1 method to pursue; identified resources, costs, & practical approach • 2/22, Have started implementing modifications • 3/15, Have established a new prototype & begun refining • 3/29, Obtained final design prototype • 4/5 , Completing final reports, presentations, documentation

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