chemical synthesis of dna by phosphoramidite method

1. DNA/RNA Synthesis Reagents Common RNA Synthesis Dyes Phosphoramidites Labels Phosphoramidites Sensitive DNA/RNA Synthesis Synthetic DNA/RNA are important research tools in therapeutic DNA/RNA area. There are four major chemical synthetic methods of DNA/RNA: 1.Contemporary H-phosphonate method 2.Phosphodiester method 3.Phosphotriester method 4.Phophitetriester method In which, phosphitetriester method is commonly used for DNA/RNA solid-phase synthesis nowadays. In phosphitetriester method, DNA/RNA chemical synthesis is carried out by a stepwise addition, the synthesis process contains four chemical reactions: Step 1: De-blocking Step 2: Coupling Step 3: Oxidation Step 4: Capping    

2. Figure 1. Oligonucleotide synthesis from phosphoramidites. As DNA/RNA contain at least one -OH group, the introduction of the suitable protecting groups enables the oligo to convert to the respective phosphoramidite, and then it can couple the latter. Therefore, phosphoramidite coupling reaction makes phophoramidites the most important building blocks in DNA/RNA synthesis. We can provide a variety of phosphoramidites for common RNA synthesis and sensitive DNA/RNA synthesis. Why choose us? Quick response for your inquiry Experienced experts Strict QA and QC High guaranteed yields Rush service is available Completive price Comprehensive analysis Customized package Easy ordering Fast delivery           Ref:

3. 1.Hughes, R. A., Miklos, A. E., & Ellington, A. D. (2011). Gene Synthesis. Synthetic Biology, Part B - Computer Aided Design and DNA Assembly, 277–309. https://rna.bocsci.com/products-services/dna-rna-synthesis-reagents.html