1 / 28

Experiment Questions

Experiment Questions. DNA Isolation. DNA Isolation. For what purpose did you use washing-up liquid while extracting DNA from plant tissue ? To break down membranes. DNA Isolation. For what purpose did you use freezer-cold ethanol while extracting DNA from plant tissue ?

brian-collins
Download Presentation

Experiment Questions

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Experiment Questions DNA Isolation

  2. DNA Isolation For what purpose did you use washing-up liquid while extracting DNA from plant tissue ? To break down membranes

  3. DNA Isolation For what purpose did you use freezer-cold ethanol while extracting DNA from plant tissue ? To separate (or see) the DNA

  4. DNA isolation For which purpose did you use washing-up liquid or other detergent while extracting DNA from plant tissue? To break down membranes

  5. DNA Isolation For which purpose did you use freezer-cold ethanol while extracting DNA from plant tissue. To separate (or see) the DNA

  6. DNA Isolation In one of your laboratory activities you isolated DNA from a plant tissue. Where in plant cells is DNA found? Nucleus OR Chloroplasts OR Mitochondria

  7. DNA Isolation It is usual to chop the tissue and place it in a blender. (i) Suggest a reason for this. (ii) For how long should the blender be allowed to run? (i) Break up of cell (walls) or release of cytoplasm (ii) 3 seconds only

  8. DNA Isolation Washing-up liquid is normally used in this experiment. What is its function? To break down membrane(s) or membrane components

  9. DNA Isolation Explain why Sodium chloride (salt) is used. Clumps (protects) DNA / To remove protein / Separates DNA / Separates protein

  10. DNA Isolation (i) What is a protease enzyme? (ii) Why is a protease enzyme used in this experiment? (i) Breaks down (acts on) protein (ii) Proteins are associated with DNA or chromosomes

  11. DNA Isolation The final separation of the DNA involves the use of alcohol (ethanol). Under what condition is the alcohol used? Ice cold

  12. DNA Isolation For which purpose did you use washing-up liquid or other detergent while extracting DNA from plant tissue? Detergent breaks down membranes

  13. DNA Isolation For which purpose did you use freezer-cold ethanol while extracting DNA from plant tissue? To separate (or see) the DNA

  14. DNA Isolation Having obtained a plant tissue e.g. onion, kiwi, etc. (i) What was the first procedure that you followed? (ii) What was the reason for that procedure? (i) Chop (ii) To disrupt structure (or described) or to increase surface area

  15. DNA Isolation Washing-up liquid is used in the isolation. Give a reason for its use. To disrupt membranes

  16. DNA Isolation Salt (sodium chloride) is used in the isolation. Give a reason for its use. To clump the DNA (or described) or to protect DNA from other positive ions

  17. DNA Isolation (i) What is a protease? (ii) Why is a protease necessary when isolating DNA? (i) An enzyme that digests protein (ii) Because DNA is combined with protein

  18. DNA Isolation The final stage of the isolation involves the use of freezer-cold ethanol. (i) Describe how it is used. (ii) For what purpose is it used? (i) Added slowly down the side of the test tube (ii) To bring the DNA out of solution

  19. DNA Isolation Give one reason why you first chopped the plant material into very small pieces. To increase surface area or to burst cells or to break cell walls

  20. DNA Isolation Detergent and salt were added to the chopped plant material, which was then heated. How was this mixture heated? In a water bath

  21. DNA Isolation Detergent and salt were added to the chopped plant material, which was then heated. Why was this mixture heated? To inactivate enzymes

  22. DNA Isolation Later in the activity the mixture was blended for a maximum of 3 seconds. What would happen to the DNA if the mixture was blended for longer than 3 seconds? It would be shredded

  23. DNA Isolation Protease was added to the mixture. Why was protease added? To digest the protein (in the chromosomes)

  24. DNA Isolation The heated mixture of plant cells, detergent and salt was blended and then filtered. After filtration, where was the DNA of your plant tissue to be found? In the filtrate

  25. DNA Isolation What should you do after the filtration to make the DNA visible? Add freezer-cold ethanol or alcohol

  26. DNA Isolation Detergent and salt were added to the chopped plant material, which was then heated. Explain why the detergent was used. To break down cell membranes or to burst cells

  27. DND Isolation What plant did you use? Onion or Kiwi or other suitable plant

  28. DNA Isolation Where in plant cells is DNA found? Nucleus or chloroplasts or mitochondria

More Related