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NSF-ITR Meeting 10-05 Miniaturized Sensors Air molecules aerosols Water molecules

NSF-ITR Meeting 10-05 Miniaturized Sensors Air molecules aerosols Water molecules cells, viruses. Sensor Operation Sensor Specific Initial event Transduction to a signal Measurement of signal Communication of result. Initial Event Binding of analyte

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NSF-ITR Meeting 10-05 Miniaturized Sensors Air molecules aerosols Water molecules

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  1. NSF-ITR Meeting 10-05 Miniaturized Sensors Air molecules aerosols Water molecules cells, viruses

  2. Sensor Operation Sensor Specific Initial event Transduction to a signal Measurement of signal Communication of result

  3. Initial Event Binding of analyte Interrogation of free analyte Chemical reaction

  4. Binding of Analyte Gas Chemical or physical adsorption Metal oxides & semiconductors Biologicals Antibodies Nucleic acids Ligands

  5. Interrogation of free analyte Gas Ionization Spectroscopy Light, microwave, mass Water Spectroscopy Light, microwave, mass

  6. Transduction to a Signal Electrical Conductance Impedance spectroscopy voltametry Light & Microwaves Absorption Fluorescence Resonance Oscillators Mechanical motion Surface acoustic waves

  7. Gas Ionization Modi, et al., Nature, 424, 171-174, 2003

  8. Gas Ionization Modi, et al., Nature, 424, 171-174, 2003

  9. Complex Mixtures Difficult to distinguish components Signal of one component can swamp others Difficult to standardize

  10. A Possible Solution Fractionate the analyte stream Differentiate molecules based on size, shape or charge On-chip family of membranes

  11. On-chip Family of Membranes • Controlled filtration characteristics Pattern membrane characteristics Biocompatible Use with or without electrophoresis

  12. Two Membrane Types Under Evaluation Cellulose Acetate Spin cast • Alumina Nanofabricated

  13. Filtration Characteristics vs Preparation

  14. Purification: DNA from Heme Membrane + electrophoresis Mohamed, et al., Chromatography A, 2005

  15. Proposed Selective Gas Sensor On-chip membrane & ionization

  16. An Antibody Based Cell Sensor Binding agent - immobilized antibody Electrical measurement - impedance

  17. Number of captured CD4+ cells vs Impedance Mishra, et al., Biosensors & Bioelcectronics, 2005.

  18. Amperometric biosensor using ion channel proteins 4 LAYERS 4. Protein: sensing component 3. Lipid membrane: provides the proper environment for the sensor proteins 2. Hydrogel: structural (localization and stability) 1. Electrode: Detection I analyte 4 3 2 1

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