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Cells

Cells. Anne Tierney, University Teacher Room 938, Boyd Orr Building a.tierney@bio.gla.ac.uk. Available through the Centre for Bioscience www.bioscience.heacademy.ac.uk/resources/projects/tierney.aspx. Intended learning outcomes. Know what constitutes a cell Describe a typical cell

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Cells

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  1. Cells Anne Tierney, University Teacher Room 938, Boyd Orr Building a.tierney@bio.gla.ac.uk Available through the Centre for Bioscience www.bioscience.heacademy.ac.uk/resources/projects/tierney.aspx

  2. Intended learning outcomes • Know what constitutes a cell • Describe a typical cell • Prokaryote • Eukaryote • Calibrate a light microscope • Measure cells using a light microscope

  3. What is a cell? • Get into groups of two or three • You have five minutes to think of everything you can that defines what a cell is • Feed back to the class

  4. Prokaryote Simple 1-2μm No nucleus No organelles Single chromosome Types of cell - prokaryote

  5. The most common shapes of prokaryotes • Cocci – round • Bacilli – rod-shaped • Spiral

  6. Eukaryote Complex 5-100μm Membrane-bound nucleus Several types of organelle Several chromosomes Single-celled organisms Multi-cellular organisms Types of cell - eukaryote

  7. A eukaryotic cell - animal

  8. A eukaryotic cell - plant

  9. How do we measure cells? • Cells are (usually) too small to see with the naked eye • Visualised with a microscope • How can we measure with a microscope? • Done indirectly • Comparing a known scale with a scale that can be calibrated

  10. Measuring cells • eyepiece graticule • stage micrometer

  11. How do we do it? • We compare the known scale (stage micrometer) to the scale that is to be calibrated

  12. Calibrating the eyepiece graticule • The eyepiece scale is UNKNOWN • The stage scale is KNOWN • 100 stage divisions = 10mm • Calibration must be done for every magnification

  13. Calibrating the eyepiece graticule • 100 eyepiece divisions = ____ *stage divisions • We know that 100 stage divisions = 10mm • 1 stage division = ____mm • ____ *stage divisions = ____mm • 100 eyepiece divisions = ____mm • 1 eyepiece division = ____ mm or ____μm • Repeat this for each magnification

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