1 / 6

T cell ontology

T cell ontology. Penny Morel 5/13/08. General issues . Introduce “thymocyte” to replace immature Make definitions more precise. DN1 thymocyte - CD4 - CD8 - CD44 + CD25 - Introduce the concept of naïve versus antigen experienced T cell - instead of “mature”

butch
Download Presentation

T cell ontology

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. T cell ontology Penny Morel 5/13/08

  2. General issues • Introduce “thymocyte” to replace immature • Make definitions more precise. DN1 thymocyte - CD4- CD8- CD44+ CD25- • Introduce the concept of naïve versus antigen experienced T cell - instead of “mature” • Add additional T helper subsets - introduce transcription factors as part of the definition

  3. T cell development Pre T cell (ETP) Thymocyte immature T cell located in the thymus DN1 thymocyte CD4- CD8- CD44+ CD25- DN2 thymocyte CD4- CD8- CD44+ CD25+ DN3 thymocyte CD4- CD8- CD44lo CD25+ pre TCR+  T cell DN4 thymocyte CD4- CD8- CD44- CD25- pre TCR+ DP thymocyte CD4+ CD8+ TCRlo undergoing positive selection DP thymocyte CD4+ CD8+ TCRhi undergoing negative selection SP thymocyte CD4+ CD8- TCRhi SP thymocyte CD4- CD8+ TCRhi NKT cell TCR+ NK1.1 (CD56)+ CD1 restricted Natural Treg CD4+ CD8- TCRhi CD25+ FoxP3+

  4. Peripheral T cells Naïve T cell CD4+ CCR7+ CD62L+ not exposed to antigen; blood and lymphoid organs CD8+ CCR7+ CD62L+ IL-7R+ not exposed to antigen; blood and lymphoid organs CD4+ CD25+ FoxP3+ CD62L+ not exposed to antigen; blood and lymphoid organs (nTreg) Effector T cell CD4+ CCR7- CD62L- antigen experienced; blood & peripheral tissues (helper) CD8+ CCR7- CD62L- IL-7R- antigen experienced; blood & peripheral tissues (cytotoxic perforin and granzyme positive) Memory T cell Effector Memory T cellCD4+ CCR7- CD62L- IL-7R+ Ag experienced; blood & periphery CD8+ CCR7- CD62L- IL-7R+ Ag experienced; blood & periphery Central Memory T cellCD4+ CCR7+ CD62L+ IL-7R+ Ag experienced; blood & lymphoid organs CD8+ CCR7+ CD62+- IL-7R+ Ag experienced; blood & lymphoid organs

  5. Types of Effector T cells (helper) T helper 1 (Th1): CD4+ T-bet+ produce IFN-; cellular immunity to intracellular pathogens; DTH T helper 2 (Th2): CD4+ GATA-3+ produce IL-4; humoral immunity; immunity to extracellular parasites T helper 17 (Th17): CD4+ RORT produce IL-17; cellular immunity; immunity to extracellular bacteria T reg 1 (Tr1): CD4+ produce IL-10; (no signature TF) inhibit immune responses Induced Treg (iTreg): CD4+ CD25+ FoxP3+ produce TGF, IL-10; inhibit immune responses

  6. Types of effector T cells (cytotoxic) Cytotoxic T cells (CTL): CD8+ perforin+ granzyme B+;kill infected targets Tc1: CD8+ T-bet+ Eomes+ produce IFN- kill infected targets Tc2: CD8+GATA-3+ produce IL-4. IL-13; less cytotoxic Tc17: CD8+ ?RORT+ produce IL-17; ?cytotoxic Treg: CD8+ FoxP3+ suppress immune responses

More Related