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Proteomics of Cartilage and Bone Tissue Engineering

Proteomics of Cartilage and Bone Tissue Engineering. DAVID PERIS PUCHOL MÒDUL GENÒMICA I PROTEÒMICA. 1. INTRODUCTION.

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Proteomics of Cartilage and Bone Tissue Engineering

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  1. Proteomics of Cartilage and Bone Tissue Engineering DAVID PERIS PUCHOL MÒDUL GENÒMICA I PROTEÒMICA

  2. 1. INTRODUCTION • “Tissue Engineering is an interdisciplinary field that applies the principles of engineering and life sciences toward the development of biological substitutes that restore, maintain, or improve tissue function or a whole organ” Langer, R & Vacanti JP, Tissue engineering. Science 260, 920-6; 1993.

  3. 2. Tissues CARTILAGE BONE

  4. 3. Cells

  5. 4. Proteomics

  6. 4. Proteomics • MALDI TOF-TOF · High sensitivity · High resolution · Partially Quantification · Sequentiation • ESI · Sequentiation

  7. 4. Proteomics

  8. 5. Proteomic approach • Characterisation

  9. 5. Proteomic approach • Applications: 1. Basic science of bone, bone cells, cartilage and chondrocytes 2. Biomarker analyses of serum, synovial fluid and synovium 3.MS analysis of post-translational modifications: · Glycosylation · Phosphorylation · Sulfation · Ubiquitinylation and sumoylation

  10. 5. Proteomic approach • Differentiation MORPHOLOGY AP MINERALISATION

  11. 5. Proteomic approach

  12. 5. Proteomic approach

  13. 5. Proteomic approach • IMS- Imaging Mass Spectrometry

  14. 5. Proteomic approach

  15. 5. Proteomic approach

  16. 5. Proteomic approach

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