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Mycology Opportunistic Molds

Please click audio icon to hear Carol’s narration. Mycology Opportunistic Molds. Division of Medical Technology Carol Larson MSEd, MT(ASCP). Click icon for audio. Basic Characteristics. Rapid growers (4-5 days) Found in soil (saprobic) Conidia are airborne Opportunistic pathogens

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Mycology Opportunistic Molds

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  1. Please click audio icon to hear Carol’s narration MycologyOpportunistic Molds Division of Medical Technology Carol Larson MSEd, MT(ASCP)

  2. Click icon for audio Basic Characteristics • Rapid growers (4-5 days) • Found in soil (saprobic) • Conidia are airborne • Opportunistic pathogens • Treatment

  3. Click icon for audio Opportunistic Mold Classification • Aseptate (sparsely septate) / hyaline • Septate / dematiaceous • Septate / hyaline

  4. Click icon for audio Aseptate (sparsely septate) hyaline hyphae Zygomycetes • Lid lifters • Sporangiospores • Rhizoids

  5. Click icon for audio Aseptate (sparsely septate) hyaline hyphae Clinical Significance • Causes Zygomycosis (Mucormycosis, Phycomycosis) • Predisposition • Diabetics • Malnutrition • Immunosuppressed • Prolonged antibiotic therapy • Pathogenesis • Nasal sinuses • Migrate to brain and meninges

  6. Click icon for audio Aseptate (sparsely septate) hyaline hyphae Absidia species • Internodal rhizoids

  7. Click icon for audio Aseptate (sparsely septate) hyaline hyphae Mucor species • No rhizoids

  8. Click icon for audio Aseptate (sparsely septate) hyaline hyphae Rhizopus species • Nodal rhizoids

  9. Click icon for audio Aseptate (sparsely septate) hyaline hyphae In Review … • Aseptate (sparsely septate) hyaline hyphae • Zygomycetes • Lid lifters, sporangiospores • Absidia – internodal rhizoids • Mucor – no rhizoids • Rhizopus – nodal rhizoids • Zygomycosis

  10. Click icon for audio Opportunistic Mold Classification • Aseptate (sparsely septate) / hyaline • Septate / dematiaceous • Septate / hyaline

  11. Click icon for audio Septate / dematiaceous hyphae Alternaria species • Colony morphology: • Microscopic morphology:

  12. Click icon for audio Septate / dematiaceous hyphae Cladosporium species • Colony morphology: • Microscopic morphology:

  13. Click icon for audio Septate / dematiaceous hyphae In Review … • Septate, dematiaceous hyphae • Alternaria species • Cladosporium species • Generally nonpathogenic

  14. Click icon for audio Opportunistic Mold Classification • Aseptate (sparsely septate) / hyaline • Septate / dematiaceous • Septate / hyaline

  15. Click icon for audio Septate / hyaline hyphae Aspergillus species • Causes Aspergillosis • Predisposed • Debilitated & immunocompromised • Pathogenesis • Inhale conidia • Pulmonary disease • Systemic

  16. Click icon for audio Septate / hyaline hyphae Aspergillus fumigatus • Colony morphology: • Microscopic morphology:

  17. Click icon for audio Septate / hyaline hyphae Aspergillus niger • Colony morphology: • Microscopic morphology:

  18. Click icon for audio Septate / hyaline hyphae Aspergillus flavus • Colony morphology: • Microscopic morphology:

  19. Click icon for audio Septate / hyaline hyphae Aspergillus species • Colony morphology: • Velvety, white, green yellow, cinnamon brown, reverse varies • Microscopic morphology: • Single or double row of phialides covering entire vesicle

  20. Click icon for audio Septate / hyaline hyphae In Review … • Aspergillus species • A. fumigatus • A. niger • A. flavus • Many others • Aspergillosis

  21. Click icon for audio Septate / hyaline hyphae Other Opportunistic Molds • Penicillium species • Paecilomyces species • Fusarium species • Sepedonium species

  22. Click icon for audio Septate / hyaline hyphae Penicillium species • Colony morphology: • Microscopic morphology:

  23. Click icon for audio Septate / hyaline hyphae Paecilomyces species • Colony morphology: • Microscopic morphology:

  24. Click icon for audio Septate / hyaline hyphae Fusarium species • Colony morphology: • Microscopic morphology:

  25. Click icon for audio Septate / hyaline hyphae Sepedonium species • Colony morphology: • Microscopic morphology:

  26. Click icon for audio Septate / hyaline hyphae In Review … • Septate hyaline hyphae • Opportunistic pathogens • Aspergillus • Fusarium • Lab contaminants • Penicillium • Paecilomyces • Sepedonium

  27. Click icon for audio Opportunistic Molds In Summary … • Key characteristics • Identification methods • Colony morphology • Microscopic morphology • Clinically significant opportunistic molds • Zygomycetes • Aspergillus fumigatus, A. niger, A. flavus • Fusarium species

  28. Who am I? Potato Dextrose Agar LPCB Stain of Slide Culture Aspergillus fumigatus

  29. Who am I? Potato Dextrose Agar LPCB Stain of Slide Culture Rhizopus species

  30. Who am I? Potato Dextrose Agar LPCB Stain of Slide Culture Fusarium species

  31. Who am I? Potato Dextrose Agar LPCB Stain of Slide Culture Penicillium species

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