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Biochemical Mechanisms for Long-term Information Storage at the Cellular Level

Biochemical Mechanisms for Long-term Information Storage at the Cellular Level. From Mechanisms of Memory by J. David Sweatt, Ph.D. Protein Synthesis Dependence of L-LTP. Kang et al. (2001) Cell 106:771-783. PO 4. PO 4. The CREB/CRE Gene Regulation System. acetylation. pKAF. Histone.

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Biochemical Mechanisms for Long-term Information Storage at the Cellular Level

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  1. Biochemical Mechanisms for Long-term Information Storage at the Cellular Level From Mechanisms of Memory by J. David Sweatt, Ph.D.

  2. Protein Synthesis Dependence of L-LTP Kang et al. (2001) Cell 106:771-783.

  3. PO4 PO4 The CREB/CRE Gene Regulation System acetylation pKAF Histone CREB Binding Protein (CBP) RNA Helicase A ser 133 ser 133 RNA Polymerase II Basal Transcription Factor IID (TFIID) Complex CREB CREB Target Gene cAMP Response Element 5’-TGACGTCA-3’ TATA Sequence

  4. Crystal Structure of CREB bound to the CRE Figure courtesy of Jennifer Gatchel Brookhaven National Protein Structure Data Bank, Rendered with Rasmol

  5. Activity-dependent Regulation of Gene Expression

  6. Transcriptional Regulation Pathways CaMKIV + ERK RSK2 Elk-1 CBP/CREB zif268 CRE SRE C/EBP krox20 Arc AMPAR HOMER BDNF NT-3 MKP-1 SSAT

  7. Activity-dependent Arc Expression and Dendritic Localization Steward and Worley (2001) PNAS 98:7062-7068.

  8. Hypothetical Mechanisms for Activity-dependent changes in Synaptic Structure

  9. - - Signaling Mechanisms Used by Integrins and Cadherins Presynaptic Integrins Ca++ Ca++ Cadherins Integrins α α α-actinin Catenins talin β β rho rac α-actinin vinculin Paxillin DMPK actin FAK (Focal Adhesion Kinase) Myosin Phosphatase grb2/sos cas/crk src Cytoskeletal Myosin II ? Dynamic cytoskeletal regulation ERK JNK Postsynaptic

  10. L-LTP Decay is an Active Process Villarreal et al. (2002). Nat. Neurosci. 5:48-52.

  11. Neurogenesis in the Adult CNS Trace Eyeblink Delay Eyeblink Morris Watermaze Post training Injection Gould et al. (1999) Nat. Neurosci. 2:260-265.

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