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Class Topics

Explore the fascinating world of DNA fingerprinting and cloning in the production of genetically modified drugs. Learn about the ethical issues surrounding these advancements and their impact on society.

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Class Topics

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  1. Complete video “Harvest of Fear” GM drugs Cloning DNA fingerprinting "Far better it is to dare mighty things, to win glorious triumphs even though checkered by failure, than to rank with those poor spirits who neither enjoy nor suffer much because they live in the gray twilight that knows neither victory nor defeat." Theodore Roosevelt To learn about DNA fingerprinting. Title: Biology 4/20/07 Class Topics Objectives: Wednesday, January 8, 20202:22 AM

  2. Class Assignments What By When • Chapter 11 Quiz/Essay 4/24/07 • Due this class period • Due next class period • Due in the future

  3. Grade Sheet 2A – p. 157 (5 pts.)

  4. Essay possibilities • Introduction • What are you going to cover? • Limit pronouns • 4 body paragraphs • 1. What is a GMO? • 2. How are GMOs made? • 3 & 4 opinion statements (on next slide) • Conclusion • What did you cover?

  5. Body paragraphs 3 and 4 • Opinion statements on… • What do you think the government should do about potential resistant weeds or insects due to excessive use of Bt or Round up Ready crops? • Do you think GM foods should be labeled? • Do you think GM crops should be used in developing countries? • Do you think companies, like Monsanto, should own (holds patents) genes and sell them • 3rd paragraph should be supporting your opinion with facts • 4th paragraph should be supporting opinion against arguments

  6. Drugs • GM drugs • Clotting factor proteins for hemophilia • Erythropoetin – for anemics • Human growth hormone • Insulin - diabetics

  7. From: http://esg-www.mit.edu:8001/esgbio/rdna/rdna.html#overview

  8. Cloning • Dolly the sheep in 1996 • Egg cell extracted from sheep and enucleated • Mammary cell extracted from Dolly • The two cells were shocked to stimulate fusion of the cells • Grown in culture • Implanted in uterus of sheep • Cloned sheep was born • Cloned animals

  9. From: http://www.sciencecases.org/dog_cloning/dog_cloning_notes.asp

  10. Quiz Review • Cloning • How to • Drugs • DNA fingerprint • Technique • Southern Blot • Uses • Recovering the Romanovs

  11. Ethical Issues • Good or Baad? • Problems

  12. DNA Fingerprinting • RFLPs • Restriction fragment length polymorphisms • When restriction enzymes are used to cut DNA they leave it in different size lengths • The pattern left by the RFLPs after a Southern Blot technique is a DNA Fingerprint • Used commonly in criminal cases and forensics

  13. Southern Blot technique • DNA of interest is cut by restriction enzymes • Fragments are separated by gel electrophoresis • Separates DNA by size • Smaller fragments move farther than large fragments • DNA is negatively charged, the ends of the gel are positively charged • DNA bands are transferred (blotted) onto filter paper • Radioactive tracers for visible bands

  14. DNA Fingerprinting • A Confounding Confusing Confectionery Crime • Recovering the Romanovs

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