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Time (min)

A. 2. 3. 4. Se (µg L -1 ). 1. 5. TFA. Time (min). 167. 100. B. MeSeCys. 313. 100. C. Peak 1-3. GluMeSeCys. 2. D. 80. Peak 4. 80. 60. 60. 165. Relative Abundance. 40. Se (µg L -1 ). 311. Relative Abundance. 40. 309. 1. 169. 184. 20. 315. 163. 182. 310. 20.

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Time (min)

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  1. A 2 3 4 Se (µg L-1) 1 5 TFA Time (min) 167 100 B MeSeCys 313 100 C Peak 1-3 GluMeSeCys 2 D 80 Peak 4 80 60 60 165 Relative Abundance 40 Se (µg L-1) 311 Relative Abundance 40 309 1 169 184 20 315 163 182 310 20 3 314 0 HFBA 145 155 165 175 185 195 m/z 0 306 310 314 318 322 326 m/z Time (min)

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