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A. MW(kDa). 1. 2. 3. 4. 5. 6. 7. 8. 66. ExoT. ExoS. 43. 14.3 2.3. (1). 2.2 0.5. 17.2 7.4. -. -. -. -. -. IPTG pulse. +. +. +. IPTG during Ca 2+ depletion. +. CHA-D1. CHA-D1 (p exsA ind). CHA. PAO1. PAO1 (p exsA ind). Strains. B. MW(kDa). 1. 2. 3.
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A MW(kDa) 1 2 3 4 5 6 7 8 66 ExoT ExoS 43 14.3 2.3 (1) 2.2 0.5 17.2 7.4 - - - - - IPTG pulse + + + IPTG during Ca2+depletion + CHA-D1 CHA-D1 (pexsAind) CHA PAO1 PAO1 (pexsAind) Strains B MW(kDa) 1 2 3 4 66 ExoT ExoS 43 12.6 4.1 - - IPTG Pulse + + 8H Time laps before Ca2+ depletion 6H