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BIOL 4014 Microbiology Fall 2005

BIOL 4014 Microbiology Fall 2005 Mon & Wed, Lecture 8 am LSW 444; Lab 9-10:50 am or 3-4:50 pm, LSW 546 Instructor: Dr. David F. Gilmore Office: LSE 418 Phone 972-3632

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BIOL 4014 Microbiology Fall 2005

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  1. BIOL 4014 Microbiology Fall 2005 Mon & Wed, Lecture 8 am LSW 444; Lab 9-10:50 am or 3-4:50 pm, LSW 546Instructor: Dr. David F. Gilmore Office: LSE 418 Phone 972-3632 Email: dgilmore@astate.edu Web: http://www.clt.astate.edu/dgilmore Tentative Office hours: Tuesday & Thursday 10:30- noon, Friday 8:30- 10:30; other times by appt.Text: Microbiology by R. Bauman. Lab text: A Photographic Atlas for the Microbiology Lab, 3rd ed, Leboffe and Pierce.

  2. Integrated Lab/Lecture course • Match up of lecture topics and Lab exercises • Additional time for discussion of lecture material, lecture exams. • Avoid “is this lab or lecture?” thinking. • Powerpoint lectures • Each slide numbered for your reference • Listen to ME, take smart notes • Powerpoints will be posted following class • Stuff • Schedules may change, more likely in lab • Alteration in points for Lab assignments • Don’t be a stranger. • Please no cell phone use or ringing in class.

  3. Grading • Four regular exams, 100 points each • Final (fifth) Exam, 100 points. Comprehensive final optional. • Lab assignments totalling 450 points • Two or Three Lab Reports • One Lab Exam and one Lab Final • One or two Unknown identifications • Attendance in lecture: 50 points. • Total 1000 points Student email, my web page Student ID numbers Cheating Roll call/ about attendance

  4. Microbiology: Definitions • Chapter 1 in text • Microbiology: study of living things too small to be seen w/o a microscope. • What’s life? • Highly organized, self replicating, self-adjusting, capable of evolving, can obtain energy • Are all microbes that small? • Epulopiscium and Thiomargarita: visibly large bacteria

  5. Classification of Microbes • Three domains • Eubacteria: prokaryotic cell structure • Archaebacteria: prokaryotes, but different • Eukaryotes: 4 kingdoms • Plants, animals, fungi, and protists. • What are microbiologists interested in? • Eubacteria and archaebacteria for sure. • Eukaryotes like fungi and protists • Animals (parasitic worms) but not really plants. • What’s missing? • VIRUSES!

  6. Divisions of Microbiology • By critter type: • Bacteriology, virology, mycology • Parasitology (includes protozoa, fungi, worms) • Other divisions: • Pathogenic microbiology, Immunology, molecular biology, microbial ecology • Applied microbiology: water treatment, natural products, food microbiology, environmental microbiology

  7. History: Ancient knowledge • Recognition of Immunity: • Variolation and protection from infection • Intentional contact with minor form of smallpox • Edward Jenner and cowpox • Milkmaids catch cowpox, seem to be immune to smallpox. • Contagion: disease can be spread by contact. • Exclusion of lepers; burning of plague victims • Catapulting of disease victims into castles during seige

  8. Satire on Jenner and vaccination encarta.msn.com/.../ Vaccination_with_Cowpox.html

  9. History continued • Microbiology as a biological science • Robert Hooke, 1665, discovery of cells • Antony van Leeuwenhoek, father of microbiology • Dutch amateur lens grinder • First person to see microbes, late 1600s • Mid 1800s, microbes taken more seriously and studied using the scientific method micro.magnet.fsu.edu/.../ introduction.html

  10. History: the Golden Age • From about 1850 to start of 20th century • Pasteur lays to rest the idea of spontaneous generation • Pasteur shows fermentation associated with life • Prevents unwanted fermentation by Pasteurization • Saves the French wine industry • Germ theory of disease: sicknesses caused by microbes • Robert Koch: lab techniques like agar, staining • Koch’s postulates: how to link a microbe w/ a disease. • Semmelweis and handwashing • Lister and aseptic surgery • Ehrlich and antimicrobials

  11. 20th Century Microbiology • Molecular biology • Use of microbes as model systems for study • Study of DNA, proteins synthesis • Tools and processes for recombinant DNA • Applied microbiology • Food industry • Water and sewage treatment • Bioremediation • Medicine • Emerging diseases; antibiotic resistance

  12. Why Study Microbes? • Major impact on health • Responsible for disease in humans, animals, plants • Major impact on environment • Major decomposers • Nutrient cycling, elemental cycling • Microbes are talented • Live under extreme conditions • Protect against disease • Eat oil, toxic waste (bioremediation) • Make plastic • Spoil food, make food • Use light, produce light

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