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Fundamentals of Microbiology Biol 315

Fundamentals of Microbiology Biol 315. Lab 2 Instruments and common chemicals in microbiology, Microscopy, Sterilization techniques. Common Instruments. Autoclave (121◦C, 15 psi) Oven (0-300 ◦C) Incubator (0-120 ◦C) pH Meter Hotplate and Magnetic Stirrer

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Fundamentals of Microbiology Biol 315

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  1. Fundamentals of MicrobiologyBiol 315 Lab 2 Instruments and common chemicals in microbiology, Microscopy, Sterilization techniques

  2. Common Instruments • Autoclave (121◦C, 15 psi) • Oven (0-300 ◦C) • Incubator (0-120 ◦C) • pH Meter • Hotplate and Magnetic Stirrer • Laminar Flow Cabinet / Biosafety Cabinet

  3. AUTOCLAVE

  4. http://textbookofbacteriology.net/autoclave1.jpg

  5. http://i01.i.aliimg.com/photo/v0/108072579/Autoclave_Portable_Autoclave_Laboratory_Autoclave_Laboratory_Equipments.jpghttp://i01.i.aliimg.com/photo/v0/108072579/Autoclave_Portable_Autoclave_Laboratory_Autoclave_Laboratory_Equipments.jpg

  6. http://www.naanolabindia.com/laboratory-instruments/vertical-autoclave.jpghttp://www.naanolabindia.com/laboratory-instruments/vertical-autoclave.jpg

  7. http://www.pharmaceutical-int.com/upload/image_files/articles/images/companies/865/large-capacity-autoclave-b.jpghttp://www.pharmaceutical-int.com/upload/image_files/articles/images/companies/865/large-capacity-autoclave-b.jpg

  8. http://www.theprominex.com/gallery/images/medical/autoclave.jpghttp://www.theprominex.com/gallery/images/medical/autoclave.jpg

  9. Oven

  10. http://2.imimg.com/data2/FU/BT/MY-2143786/1-250x250.jpg

  11. http://images.hellotrade.com/data/T/W/MY-633236/Hot-Air-Oven---Lab-Model_250x250.jpghttp://images.hellotrade.com/data/T/W/MY-633236/Hot-Air-Oven---Lab-Model_250x250.jpg

  12. http://faculty.ksu.edu.sa/baddour/PublishingImages/hotairoven.jpghttp://faculty.ksu.edu.sa/baddour/PublishingImages/hotairoven.jpg

  13. INCUBATORS

  14. http://www.stuart-equipment.com/adminimages/Incubators_SI505_open.jpghttp://www.stuart-equipment.com/adminimages/Incubators_SI505_open.jpg

  15. http://www.stuart-equipment.com/adminimages/Incubators_SI505_closed.jpghttp://www.stuart-equipment.com/adminimages/Incubators_SI505_closed.jpg

  16. Shaking Incubator http://www.ika.com/ika/product_art/images/3510000_1.jpg

  17. pH Meter

  18. http://growsetup.files.wordpress.com/2011/02/3505-ph-meter.jpghttp://growsetup.files.wordpress.com/2011/02/3505-ph-meter.jpg

  19. Hotplate and magnetic stirrer

  20. http://www.labdepotinc.com/admin/uploads/scilogex-ms-h-s-m-10008-vlarge-r_1.jpghttp://www.labdepotinc.com/admin/uploads/scilogex-ms-h-s-m-10008-vlarge-r_1.jpg

  21. http://www.troemner.com/images/hotplate_stirrers/adv_hps_lge.jpghttp://www.troemner.com/images/hotplate_stirrers/adv_hps_lge.jpg

  22. http://pimg.tradeindia.com/00076902/b/0/Laboratories-Magnetic-Stirrer-Hotplate.jpghttp://pimg.tradeindia.com/00076902/b/0/Laboratories-Magnetic-Stirrer-Hotplate.jpg

  23. Biosafety cabinet

  24. http://www.terrauniversal.com/gallery/hoods/Images/biological_safety_cabinet_purifier_Cover.jpghttp://www.terrauniversal.com/gallery/hoods/Images/biological_safety_cabinet_purifier_Cover.jpg

  25. Class I Cabinets • These cabinets have unrecirculated airflow away from the operator that is discharged to the atmosphere after filtration through a HEPA filter. • They provide good operator protection but do not protect the material within the cabinet (the product) from contamination.

  26. Class II Cabinets • Class II cabinets are designed for personnel, product and environmental protection. • They are designed for work involving microorganisms in containment levels 2, 3 and 4 laboratories. • Class II cabinets are most commonly used in biomedical research laboratories because of their characteristics.

  27. http://escoglobal.com/products/images/products-big/1322810342.jpghttp://escoglobal.com/products/images/products-big/1322810342.jpg

  28. Class III Cabinets • Class III cabinets are totally enclosed and gas-tight with HEPA filtered supply and exhaust air. Work is performed with attached long-sleeved gloves. • The cabinet is kept under negative pressure and airflow is maintained by a dedicated exterior exhaust system. • Class III cabinets protect the worker and the product. They are designed for work with level 4 pathogens. • The exhaust air is double HEPA filtered or treated by HEPA filter and incineration.

  29. http://www.phac-aspc.gc.ca/publicat/lbg-ldmbl-04/gfx/fig6_e.gifhttp://www.phac-aspc.gc.ca/publicat/lbg-ldmbl-04/gfx/fig6_e.gif

  30. http://doh.sd.gov/img/bsc.jpg

  31. http://www.nap.edu/books/0309075718/xhtml/images/p20005395g49001.jpghttp://www.nap.edu/books/0309075718/xhtml/images/p20005395g49001.jpg

  32. http://escoglobal.com/products/images/products-big/AC2-D.jpg

  33. http://web.princeton.edu/sites/ehs/biosafety/biosafetypage/BSC2.jpghttp://web.princeton.edu/sites/ehs/biosafety/biosafetypage/BSC2.jpg

  34. Chemicals • Media (solid and liquid) • Nutrient agar, nutrient broth • EMB agar, MacConkey’s agar, Blood agar • Lactose broth • Gram’s staining kit • Catalase reagent • Oxidase reagent

  35. Microscopy • Light microscope • Photography and video making through microscope using live camera • Other kinds of microscopy

  36. Gram’s Staining result

  37. http://janiceatmeredith.files.wordpress.com/2012/01/gramsta.gifhttp://janiceatmeredith.files.wordpress.com/2012/01/gramsta.gif

  38. THANK YOU

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