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Histology Laboratories Molecules to Systems 2003 Compiled by James D. Jamieson, MD/PhD

Histology Laboratories Molecules to Systems 2003 Compiled by James D. Jamieson, MD/PhD Thomas L. Lentz, MD. No part of this image collection may be distributed outside of the Yale University Intranet. Acknowledgements. Sources of Micrographs, Diagrams and Figures.

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Histology Laboratories Molecules to Systems 2003 Compiled by James D. Jamieson, MD/PhD

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  1. Histology Laboratories Molecules to Systems 2003 Compiled by James D. Jamieson, MD/PhD Thomas L. Lentz, MD No part of this image collection may be distributed outside of the Yale University Intranet.

  2. Acknowledgements Sources of Micrographs, Diagrams and Figures Alberts, B. et al. Molecular Biology of the Cell. 4th Edition, Garland Science, New York, 2002. Gartner, L. P. and Hiatt, J. L. Color Atlas of Histology, Williams & Wilkins, Baltimore, 1994. Kerr, J. B. Atlas of Functional Histology. Mosby, London, 1999. Kessel, R. G. and Kardon, R. H. Tissues and Organs: a text-atlas of scanning electron microscopy. W. H. Freeman, San Francisco, 1979. Lentz, T. L. Cell Fine Structure. W. B. Saunders, Philadelphia, 1971. Lodish, H. et al. Molecular Cell Biology. W. H. Freeman, New York, 2000. Mizoguti, H. Color Slide Atlas of Histology. Nihon Shashin Shinbunsha, Tokyo. Young, B. and Heath, J. W. Wheater’s Functional Histology. Churchill Livingstone, Edinburgh, 2000. Micrographs taken by George Palade, Marilyn Farquhar, James D. Jamieson, Nicolai Simionescu, Maya Simionescu, David Castle, Thomas L. Lentz. Web Resources http://info.med.yale.edu/webpath/webpath.htm Cushing Library Educational Software/Cell Biology/Several Histology Resources

  3. Urinary System Laboratory

  4. Some Handy Abbreviations G: glomerulus PCT: proximal convoluted tubule tDL: thin descending limb of Henle tAL: thin ascending limb of Henle TAL: thick ascending limb of Henle DCT: distal convoluted tubule (similar to TAL) CT: connecting/collecting tubule CD: collecting duct MD: macula densa AA: afferent arteriole (in) EA: efferent arteriole (out)

  5. Cortex BV RT G CD Medulla Renal Cortex and Medulla Vasa recta

  6. Organization of Renal Pyramid

  7. The Nephron

  8. Renal Tubules

  9. Renal Corpuscle (Glomerulus, afferent and efferent arterioles, Bowman’s capsule, beginning of proximal convoluted tubule)

  10. Diagram of Renal Corpuscle/Glomerulus

  11. Renal Corpuscle/Glomerulus

  12. Development of Renal Corpuscle

  13. PCT CD G DCT Renal Corpuscles

  14. Bowman’s space G Bowman’s capsule parietal layer visceral layer PCT Renal Corpuscles

  15. MD Vascular pole Bowman’s capsule parietal layer visceral layer G Bowman’s space PCT Urinary pole Renal Corpuscle

  16. Podocytes Bowman’s capsule parietal layer visceral layer PCT Bowman’s space (urinary space) Mesangial cells DCT Renal Corpuscle

  17. Beginning of PCT Urinary Pole of Renal Corpuscle

  18. Glomerular Filter

  19. Bowman’s space Lumen of glomerular capillary Podocyte Foot processes of podocytes Fenestrae Urinary space Urinary space Glomerular BM Fenestrated Capillaries in Glomerulus

  20. Podocyte FS: filtration slits Glomerulus

  21. Filtration slits with diaphragms Capillary space Urinary space Components of Glomerular Filter

  22. Scanning EM of Glomerulus Foot processes Filtration slits

  23. Renal Tubules in Sequence Proximal Convoluted Tubules (PCT): Epithelium: brush border; basal infoldings Loop of Henle: straight portion of PCT (or thick descending limb) (TDL)>thin descending limb (tDL)>loop>thin ascending limb (tAL)>straight portion of DCT (or thick ascending limb) (TAL) Epithelium: Simple squamous epithelium on thin segments; epithelium similar to PCT and DCT on thick segments Distal Convoluted Tubule: Continuous with TAL>macula densa>DCT Epithelium: No brush border, numerous mitochondria Collecting Tubule: Epithelium: cuboidal Collecting Duct: Epithelium: cuboidal

  24. Brush border Proximal Convoluted Tubule

  25. Brush border Proximal Convoluted Tubule

  26. M: mitochondria Mv: microvilli of brush border Proximal Convoluted Tubule

  27. PCT Vasa recta T T CD Thin Walled Tubules of Loop of Henle

  28. VR TAL tL TAL tL CD Relationhship Between CD, TAL, tL and Vasa Recta (VR)

  29. Basolateral infoldings with mitochondria Distal Convoluted Tubule

  30. Basolateral infoldings with mitochondria; few microvilli Distal Convoluted Tubule

  31. Collecting Ducts, LS

  32. Collecting Ducts, XS

  33. Site of urine outflow at renal papilla Renal pelvis Apex of Renal Pyramid

  34. Countercurrent Multiplier

  35. Countercurrent Multiplier

  36. Countercurrent Multiplier

  37. Juxtaglomerular Apparatus and Control of Blood Pressure and Volume

  38. JGA and Control of Blood Pressure

  39. Diagram of JG Apparatus

  40. AA: afferent arteriole J: juxtaglomerular cells MD: macula densa DCT: distal conv. tubule JG Apparatus

  41. DT JG Apparatus: Mg, mesangial cells; P, podocyte; JC, juxtaglomerular cells with secretory granules containing renin (arrowheads)

  42. Ureter, Bladder and Urethra

  43. Transitional epithelium Smooth muscle Ureter

  44. Transitional epithelium Relaxed Bladder

  45. Distended Bladder

  46. Male Urethra: transitional > stratified columnar epithelium

  47. Some Pathology

  48. Normal Glomerulus and Tubules PAS stain for basement membrane glycoproteins.

  49. Diabetic Kidney PAS stain. What component of the filtration apparatus might you see altered in an EM?

  50. Membranous Glomerulonephritis What structure is the membranous change affecting? The next EM taken at biopsy cinches the diagnosis.

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