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aerobic – require oxygen. 3 parts of Respiration. Glycolysis – may be anaerobic TCA – Kreb’s Cycle Electron Transport Chain. Electron Transport Chain. a.k.a cytochrome chain Main ATP producer! Chemiosmotic theory explains how the cytochrome chain produces ATP
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aerobic – require oxygen 3 parts of Respiration • Glycolysis – may be anaerobic • TCA – Kreb’s Cycle • Electron Transport Chain
Electron Transport Chain • a.k.a cytochrome chain • Main ATP producer! • Chemiosmotic theory explains how the cytochrome chain produces ATP • Step 1: creating a H+ concentration gradient • Step 2: using the gradient to make ATP
Creating a Potential • Carrier molecules (cytochromes) in the inner membrane transport H+ out; accumulates in the intermembrane space • Creates an electrochemical gradient!
Click here for ETC Click here for ATP synthase close-up
Creating a Potential • NADH and FADH2bring H atoms to the chain. • e- are stripped off and passed down the chain of cytochromes • Oxygen becomes the final electron acceptor to form water.
Electron shuttles span membrane MITOCHONDRION CYTOSOL 2 NADH or 2 FADH2 2 FADH2 2 NADH 2 NADH 6 NADH Glycolysis Oxidative phosphorylation: electron transport and chemiosmosis Citric acid cycle 2 Acetyl CoA 2 Pyruvate Glucose + 2 ATP + 2 ATP + about 32 or 34 ATP by oxidative phosphorylation, depending on which shuttle transports electrons from NADH in cytosol by substrate-level phosphorylation by substrate-level phosphorylation About 36 or 38 ATP Maximum per glucose:
Anaerobic Respiration (fermentation) • Require the energy-carriers formed by glycolysis • Alcholic fermentation • Lactic Acid fermentation
Alcoholic Fermentation • Performed by yeast cells • If NADH can’t dump e- into ETC, it dumps them back into pyruvate; making it the electron acceptor! • NAD+ is restored and recycles back into glycolysis yielding only 2 ATP molecules • Pyruvate will eventually become ethanol (which is an alcohol, hence alcoholic fermentation… DUH!)
Lactic Acid Fermentation • Occurs in muscle cells • Similar to alcoholic fermentation where pyruvate becomes the final electron acceptor • Pyruvate is converted to lactic acid
Energetics of Respiration • Aerobic vs. Anaerobic: Which is more efficient? Aerobic: yields 38 ATP per glucose molecule Anaerobic: yields 2 ATP per glucose molecules • Anaerobic is not as efficient, but still allows you to live without oxygen
Alternative Food Molecules • Fats and proteins can change form that can enter normal respiration pathways • Fats • Glycerol enters glycolysis • Fatty acids into acetyl-CoA • Proteins • Deaminated, remainder enters at various points in the chain