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Autoclave

Autoclave. By: Carolina Gallango-Brun Sana Khan Vedrana Sahovic. Definition. Autoclave is a pressurized device designed to heat aqueous solutions above their boiling point at normal atmospheric pressure to achieve sterilization . Fig. 1. Autoclave machine. Brief History.

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Autoclave

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  1. Autoclave By: Carolina Gallango-Brun Sana Khan Vedrana Sahovic

  2. Definition • Autoclave is a pressurized device designed to heat aqueous solutions above their boiling point at normal atmospheric pressure to achieve sterilization Fig. 1. Autoclave machine

  3. Brief History • 1450 B.C.E. Moses • 460-377 B.C.E. Hippocrates • Lazzaro Spallanzani • Pasteur • Denis Papin Fig. 2. Papin’s steam cooker

  4. 20th Century • It was finalized by Charles Chamberland in 1987 • Colleague of Pasteur • Building up on Papin’s marmite • From culinary to medicine Fig. 3. Charles Chamberland 1851-1908

  5. How Des it Works? • Liquid H2O cannot be heated above 100°C in an open vessel • At 100°C boils • H2O heated in a sealed vessel • Pressure rises • Boiling point of H2O is raised

  6. The Process • Air removal • Downward displacement • Steam pulsing • Vacuums pumps • Superatmospheric • Subatmospheric

  7. Autoclave Usage • Microbiology • Medicine • Body piercing • Veterinary science • Dentistry • Podiatry • Metallurgy …

  8. Video Clip

  9. References • http://en.wikipedia.org/wiki/Autoclave • http://138.23.152.128/protocols/autoclave%20operation.pdf

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