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Characterization of An Immune Response in Melanoma. By: Suren Ravindran Mentor: Dr. Taha Merghoub. What is Melanoma?. Melanoma is one of the most serious and devastating types of skin cancer. Due to the uncontrolled cell division of abnormal melanocytes.
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Characterization of An Immune Response in Melanoma. By: Suren Ravindran Mentor: Dr. Taha Merghoub
What is Melanoma? • Melanoma is one of the most serious and devastating types of skin cancer. • Due to the uncontrolled cell division of abnormal melanocytes. • Melanocytes- skin cells that produce melanin (pigment). • Can spread and form tumors in other parts of the body other than the skin. (metastasis)
What is Melanoma? Cont’d Healthy Melanocytes Melanocytes in Melanoma
Procedure • Dissect transgenic mice and remove spleens. (control and tumor bearing) • Crush spleen and prepare into a suspension for flow cytometry. • Run through FACS machine and analyze data.
Immune Response • Integrated response to an antigen mediated by lymphocytes and involving recognition of antigens by specific antibodies. • Types of lymphocytes are T- Cells and B- Cells.
Flow Cytometry • Process in which the properties of cells are identified through a systematic procedure of detecting the fluorescence of the cells. • Antibodies conjugated with fluorescence is added to a cell suspension where they then attach to the specific antigens on the cells. • Cell suspension is then processed in FACS (fluorescence- activated cell sorter) machine.
Expected Results • With the data derived from the FACS machine we can determine the amount, size, granularity, and the amount of fluorescence of the cells. • With this information we can observe the “landscape” of the tumor and the composition of the immune response.
References • www.uhseast.com/128019.cfm • www.mskcc.org/mskcc/html/2468.cfm • www.pg.com/science/skincare/Skin_tws_16.htm;j... • http://runzwithscissors.net/gallery/albums/brains/MELANOMA.sized.jpg • The Melanoma Letter • What You Need To Know About Melanoma • www.mskcc.org/mskcc/print/2270.cfm?IRBNO=03-007 • http://www.fcspress.com/html/FCSPressFeatures/Plots.GIF • http://www.flow-cytometry.de/definition.html • http://en.wikipedia.org/wiki/Flow_cytometry
Acknowledgments • Dr. Taha Merghoub • Elva Barreda • Dr. Sat & Harlem Children Society • Gateway Program