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DIAGNOSTICUL Î N PSEUDOPESTA AVIAR Ă (NEWCASTLE DISEASE)

DIAGNOSTICUL Î N PSEUDOPESTA AVIAR Ă (NEWCASTLE DISEASE). Fam.Paramyxoviridae Gen. Rubulavirus 9 serotipuri APMV–I to APMV– 9 APMV – serotipul 1 ARN Velogene Tulpini :Mezogene Lentogene. Hemaglutinina Neuraminidaza. DIAGNOSTIC EPIDEMIOLOGIC CLINIC

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DIAGNOSTICUL Î N PSEUDOPESTA AVIAR Ă (NEWCASTLE DISEASE)

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  1. DIAGNOSTICUL ÎN PSEUDOPESTA AVIARĂ (NEWCASTLE DISEASE)

  2. Fam.Paramyxoviridae Gen.Rubulavirus 9 serotipuri APMV–I to APMV– 9 APMV – serotipul 1 ARN Velogene Tulpini :Mezogene Lentogene Hemaglutinina Neuraminidaza

  3. DIAGNOSTIC • EPIDEMIOLOGIC • CLINIC • ANATOMOPATOLOGIC/HISTOPATOLOGIC • CONFIRMAREA PRIN TESTE DE LABORATOR!

  4. Epidemiologie Morbiditate: pana la100% Mortalitate: 90%

  5. Tablouclinic

  6. Tablou anatomopatologic

  7. HISTOPATOLOGIC NODUL GLIAL NEURONOFAGIE , SATELITOZĂ PERIVASCULARITĂ

  8. Diagnosticul de laborator • Recoltarea de probe se face cu respectarea măsurilor de protecţie a muncii şi mediului • Materiale patologice: • cadavre întregi • organe : aparat digestiv – stomac glandular, triturator, intestin; aparat respirator: trahee, pulmon; sistem nervos: encefal • Pregătirea materialului pentru identificarea virală • triturare, omogenizare cu SF • centrifugare (6000 rpm, 45 min) • aseptizarea supernatant cu antibiotice (penicilină 1000 UI/ml, streptomicină 1000 /ml)

  9. Diagnosticul de laborator Izolarea şi identificarea virusului - reactia dehemaglutinare - HA - cultivarea pe ouă embrionate, culturi celulare - infecţia experimentală - microscopia electronică - PCR - imunohistochimia - hibridizarea in situ

  10. Diagnosticul de laborator Identificarea anticorpilor IHA – reactia de inhibare a hemaglutinarii - pentru identificarea infecţiei - pentru titrarea anticorpilor postvaccinali ELISA – directă sau indirectă

  11. HA

  12. IHA - etapa I – HA titrarea antigenului

  13. IHA - etapa II – titrarea Ac

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