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DIAGNOSTICUL Î N PSEUDOPESTA AVIAR Ă (NEWCASTLE DISEASE)

DIAGNOSTICUL Î N PSEUDOPESTA AVIAR Ă (NEWCASTLE DISEASE). Fam.Paramyxoviridae Gen. Rubulavirus 9 serotipuri APMV–I to APMV– 9 APMV – serotipul 1 ARN Velogene Tulpini :Mezogene Lentogene. Hemaglutinina Neuraminidaza. Cultivare – oua embrianate – moartea embrionilor

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DIAGNOSTICUL Î N PSEUDOPESTA AVIAR Ă (NEWCASTLE DISEASE)

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  1. DIAGNOSTICUL ÎN PSEUDOPESTA AVIARĂ (NEWCASTLE DISEASE)

  2. Fam.Paramyxoviridae Gen.Rubulavirus 9 serotipuri APMV–I to APMV– 9 APMV – serotipul 1 ARN Velogene Tulpini :Mezogene Lentogene Hemaglutinina Neuraminidaza

  3. Cultivare – oua embrianate – moartea embrionilor • Culturi celulare – efect citopatic • 4 patotipuri: • enteric asimptomatic - subclinic • Lentogenic • Subclinic - tip respirator • Mezogenic • Respiratorii si nervoase • Velogenic • Neurotropic: Respiratorii si nervoase • Vicerotropic: leziuni hemoragice intestinale Teste de virulenta Timpul mediu de mortalitate al embrinilor de 9 zile (Mean Death Time in Eggs -OIE) Indicele de patogenitate intracerebrala la pui de 6 saptamani (Intracerebral Pathogenity Index - OIE) Indicele de patogenitate intravenoasa (Intravenous Pathogenity Index - OIE)

  4. Epidemiologie Morbiditate: pana la100% Mortalitate: 90%

  5. TablouClinic

  6. TablouAnatomopatologic

  7. Diagnosticul de laborator HA – reactia de hemaglutinare IHA – reactia de inhibare a hemaglutinarii ELISA PCR IMUNOHISTOCHIMIA HIBRIDIZAREA IN-SITU

  8. HA

  9. IHA

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